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Plasmid#97144PurposeFor Gal4/UAS expression systemDepositorInsertGal4-Vp16
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Ncad-GFP
Plasmid#35986DepositorInsertNcad (cdh2 Zebrafish)
UseGal4 inducibleTagsEGFPExpressionMutationPromoter14xUAS-E1bAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV_PP1-mCitrine
Plasmid#172472PurposeMammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.DepositorInsertprolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
UseLentiviralTagsmCitrine and prolactin signal sequence (cleaved p…ExpressionMammalianMutationPromoterEF1alphaAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-BiNLS-fYap
Plasmid#176623Purposeexpresses optofYap in zebrafishDepositorInsertYes associated protein (yap1 Zebrafish)
UseZebrafish expressionTagsmCherryExpressionMutationPromoterSP6Available sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
Plasmid#105643PurposeCo-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermisDepositorInsertKrt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-gcry-TagBFP
Plasmid#117771PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsert3x3 Stop sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; gcry1:BFP -0
Plasmid#173886PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME Integrin beta 1b
Plasmid#105976Purposeentry clone for gateway cloning, Full length Integrin beta 1b without stopDepositorInsertIntegrinbeta1b
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Control-cx43.4-48H-pGTag
Plasmid#117818PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsCAAXExpressionMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR 2xU6:gRNA pten, mitfa:Cas9
Plasmid#118845PurposeTargets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorUseCRISPR; Tol2TagsExpressionMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; HE1A:BFP -1
Plasmid#180008PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; HE1A: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; HE1A:BFP -2
Plasmid#180009PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; HE1A: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab32a
Plasmid#109621PurposeMuscle specific zebrafish expression of mKate2 fused to rab32aDepositorInsertrab32a (rab32a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISceI Dusp6:memGFP
Plasmid#32668DepositorInsertDusp6 promoter (dusp6 Zebrafish)
UseZebrafish transgenesisTagsEGFPExpressionMutationPromoterAvailable sinceNov. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
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actc1b-mKate2-rab14
Plasmid#109600PurposeMuscle specific zebrafish expression of mKate2 fused to rab14DepositorInsertrab14 (rab14 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7tyrgRNA
Plasmid#46761DepositorInserttyr gRNA (tyr Zebrafish)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:NRAS
Plasmid#118847PurposeExpresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertNRAS Q61R (NRAS Zebrafish, Human)
UseZebrafish expression, tol3TagsExpressionMutationNRAS Q61RPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTag-eGFP-B-actin
Plasmid#117814PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only