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Plasmid#161785PurposeGateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterBidirectional minhCMV and SFFVAvailable sinceDec. 30, 2020AvailabilityAcademic Institutions and Nonprofits only
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pLS-mP
Plasmid#81225PurposeLentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterDerived from pGL4.23Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-6xNLS-E174R/S542R (pRG232)
Plasmid#149723PurposeLentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF1974
Plasmid#143988PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTBX3 (TBX3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLS-mP-Luc
Plasmid#106253PurposeLuciferase lentivirus based enhancer assay vectorDepositorInsertLuciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterDerived from pGL4.23Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0118
Plasmid#141525PurposeLentiviral vector for overexpressing the FOXM1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ114
Plasmid#85995PurposeOne-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant regionDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermodified bovine U6-2Available sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBA439
Plasmid#85967PurposePerturb-seq vector backboneDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2-mDux
Plasmid#138320PurposeDox-inducible codon-optimized mouse DuxDepositorInsertDux (Dux Mouse)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTREAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_NEUROD1_P2A_Hygro_Barcode
Plasmid#120466PurposeBarcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertNEUROD1 (NEUROD1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV d14GFP Donor
Plasmid#31475DepositorInsertSFFV d14GFP
UseLentiviralTagsExpressionMutationDeleted 14 amino acids on the c terminus of eGFPPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMA3287
Plasmid#46883PurposeLentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylaseDepositorInsertUracil-N-glycosylase, Y147A mutant (UNG Human)
UseLentiviralTagsMycExpressionMammalianMutationY147APromoterTRE-TightAvailable sinceAug. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF1036
Plasmid#144074PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertWT1 (WT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
IGF1R_pLX307
Plasmid#98344PurposeLentiviral expression of IGF1RDepositorInsertIGF1R (IGF1R Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-miniAAVR-FLAG
Plasmid#166717PurposeVector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tagDepositorInsertminiAAVR-FLAG
UseLentiviralTagsFLAGExpressionMammalianMutationOnly expressing PKD1-3 of ectodomainPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE50
Plasmid#90389PurposePPARg/RARa - PPARg gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterDR1Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta
Plasmid#89347Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequencesDepositorInsertJurkat TCRalpha/beta alleles and CD3e and CD3z
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-FAM208A ts1
Plasmid#115871PurposeFAM208a knockdownDepositorInsertFAM208a shRNA (C3orf63 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0346
Plasmid#143397PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYT1L (MYT1L Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W
Plasmid#67977PurposeCRISPR gRNA expression vector with an improved scaffold and puro/mCherry markersDepositorInsertU6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only