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Plasmid#110739PurposePlasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only
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pRec
Plasmid#104572PurposeExpresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.DepositorInsertPTet-cas12, tetR, lacI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCDM
Plasmid#110742PurposePlasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB7WG_ASX
Plasmid#98891PurposePlant expression vector for multigene cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK7WG_ASX
Plasmid#98889PurposePlant expression vector for multigene cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSPL
Plasmid#110743PurposePlasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKL
Plasmid#110741PurposePlasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmRuby2-IRES-Clover
Plasmid#171059PurposeExpresses mRuby2 and mClover2 in the cytoplasm as negative control for FRET.DepositorInsertIRES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tTA
Plasmid#149363PurposeAAV-mediated and Cre-dependent expression of tTA under the CAG promoter.DepositorInserttTA2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK606
Plasmid#72529PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subunit with C-terminal His6 tag (AaPurLH6)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK624
Plasmid#72539PurposeProduces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)DepositorInsertshypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK625
Plasmid#72541PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)DepositorInsertshypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-MTM1
Plasmid#51614PurposeRecruitable PtdIns3P/PtdIns(3,5)P2 phosphataseDepositorInsertsUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-neo
Plasmid#32426DepositorInsertFlag-mCherry-T2A-GFP-T2A-neo
UseTagsExpressionInsectMutationPromoterActin5CAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
iRFP-FRB-Rab5
Plasmid#51612PurposeRecruiter to Rab5-positive endosomesDepositorInsertsFRB
Rab5
UseTagsiRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR78
Plasmid#90210PurposeExpresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyADepositorInsertsCcaS
Cph8
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
iRFP-FRB-Rab7
Plasmid#51613PurposeRecruiter to Rab7-positive endosomesDepositorInsertsFRB
Rab7
UseTagsiRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
aCE120
Plasmid#204491PurposeFigure 1. reporter circuitDepositorInsertsEBFP2
mKate2
EYFP
UseSynthetic BiologyTagsnoneExpressionBacterial and MammalianMutationPromoterhEF1aAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA_ASX
Plasmid#98888PurposePlant expression vector for multigene cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterCaMV35S (enhanced)Available sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrig-CopA
Plasmid#104578PurposeCopy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under CopA promoter for copy increase.DepositorInsertPCopA-RiboJ-B0034-repL
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only