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Plasmid#134958PurposeExpression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.DepositorInsertMyo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
UseTagsmcherryExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MITF-M (L135V)
Plasmid#31154DepositorInsertMITF-M (MITF Human)
UseTagsFLAGExpressionBacterial and MammalianMutationLeucine 135 changed to ValinePromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MITF-M (E87R)
Plasmid#31152DepositorInsertMITF-M (MITF Human)
UseTagsFLAGExpressionBacterial and MammalianMutationGlutamic acid 87 changed to ArgininePromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MITF-M (G244R)
Plasmid#31155DepositorInsertMITF-M (MITF Human)
UseTagsFLAGExpressionBacterial and MammalianMutationGlycine 244 changed to ArgininePromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-hMcl-1(T163A)
Plasmid#25376DepositorInserthMCL1 (MCL1 Human)
UseTagsV5 and His tagExpressionMammalianMutationchanged threonine 163 to alaninePromoterAvailable sinceAug. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-H-Ras_wt
Plasmid#39509DepositorInsertHRAS (HRAS Human)
UseEukaryoticTagsHAExpressionMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pet16b ALIX Bro1 (1-359)
Plasmid#80641PurposeResidues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428DepositorInsertALIX Bro1, residues 1-359 (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-359PromoterT7Available sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJSTX-GL3
Plasmid#11982PurposeReporter gene with mouse c-jun promoter and point mutation of MEF2 and AP1 binding sitesDepositorInsertc-jun promoter (Jun Mouse)
UseLuciferaseTagsLucExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJSX-GL3
Plasmid#11980PurposeReporter gene with mouse c-jun promoter and point mutation of MEF2 binding siteDepositorInsertc-jun promoter (Jun Mouse)
UseLuciferaseTagsLucExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(WT)
Plasmid#83505PurposeBacterial expression of CalreticulinDepositorInsertCALR (Calr Mouse)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:Lucia:bgH
Plasmid#135293PurposeTranscriptional unit for constitutive expression of Lucia luciferase in mammalian cellsDepositorInsertCMV:Lucia:bGH
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAL4-mNFAT1(2-144)
Plasmid#11108PurposeExpresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
UseTagsGAL4-DBDExpressionMammalianMutationContains NFAT1 amino acids 2-144; L78P and P140Q …PromoterAvailable sinceApril 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H
Plasmid#108376PurposeFRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-xrRNA-4H
Plasmid#108368PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DARPinOFF7
Plasmid#182397PurposeBacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusionDepositorInsertVNp-DARPinOFF7
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-tSapphire
Plasmid#176312Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-SapphireDepositorInsertstetR
T-sapphire
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (4x TBE2 - mut)
Plasmid#16600DepositorInsertc-myc promoter (4xTBE2 mut) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationmutant TBE2 sitesPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCI-globin_del5UTR_PTC39-xrRNA-4H
Plasmid#108367PurposeExpresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
UseTagsExpressionMammalianMutationPTC39PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only