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Plasmid#90496PurposepcDNA3 - CMVmin - PhyB - MTAD, expresses PhyB and MTAD, under CMV promoterDepositorInsertPhyB NT-MTAD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_3x_siRNA
Plasmid#59893Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sitesDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--has three siRNA-like targets (fully c…Promoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS138
Plasmid#197142PurposescCloneSelect C-to-T DNA barcode cloning backbone (CMV promoter version)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-MNTS-1-p75
Plasmid#182945PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTRDepositorUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV promoter and enhancerAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1(L106P)
Plasmid#166714Purposeexpresses AMOT(L106P) in mammalian cellsDepositorInsertAngiomotin p130 L106P (AMOT Human)
UseTags2xHAExpressionMammalianMutationchanged leucine 106 to prolinePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1(A112K)
Plasmid#166715Purposeexpresses AMOT(A112K) in mammalian cellsDepositorInsertAngiomotin p130 A112K (AMOT Human)
UseTags2xHAExpressionMammalianMutationchanged alanine 112 to lysinePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_∆R13–DD-talin1 (1-2299)
Plasmid#166125PurposeFor expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.DepositorInsert∆R13–DD-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationresidues 1-2299, deletion of dimerization domain …PromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLB(N)CX AT-CH3-FLAG-HA WY
Plasmid#133725PurposeRetroviral vector with CMV promoter driving mutant form of acetyltransferase and CH3 domains fragment of human p300 (EP300) (WY1466-1467AS in AT domain).DepositorInsertEP300 (EP300 Human)
UseRetroviralTagsFlag and HAExpressionMutationpartial (not complete) p300 fragment, AT and CH3 …PromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPKm- 227
Plasmid#90498PurposepcDNA3 - CMVmin - VPR - PIF3, expresses VPR transactivator domain and PIF3, under CMV promoterDepositorInsertVP64-PIF3
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-113
Plasmid#90495PurposepcDNA3 -pCMV - MTAD - PIF6, expresses the Phytochrome interacting factor 6 (PIF6) and MTAD, under CMV promoterDepositorInsertMTAD-PIF6
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_7x_Bulge
Plasmid#59894Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sitesDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--has seven bulge targets (fully comple…Promoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 1278-1291
Plasmid#59830PurposeAllows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 1278-1291Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
T7-Phi6-P2
Plasmid#182535PurposeTemplate for production of Phi6 P2 RNADepositorInsertP2 pol
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-puro
Plasmid#45561DepositorInsertPuromycin N-acetyl transferase
UseBifunctional selection proteinTagsEGFPExpressionMammalianMutationPromoterPhcmv (Strong human cytomegalovirus immediate ear…Available sinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.1-IGLC2-MscI
Plasmid#80797PurposeExpression of immunoglobulin light chains in mammalian cells, human lambda 2 constant regionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5E-ESyn1
Plasmid#80804PurposehSyn1 promoter fused to CMVminDepositorInsertp5E-ESyn1
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Twinkle-APEX2-V5
Plasmid#129705PurposeAPEX2 fused to the mitochondrial nucleoid protein TwinkleDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HRE-dUnaG
Plasmid#124372PurposeUnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signalingDepositorInsertHRE-dUnaG
UseLentiviralTagsPEST-degron and Myc tagExpressionMutationPromoterHRE (HIF responsive element 5x + CMV minimal prom…Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only