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Plasmid#206214PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only
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PAGFP_NS2B3
Plasmid#195755PurposeCloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.DepositorInsertDengue (Type-2) NS2B3
UseTagsPA-GFPExpressionMammalianMutationNo mutationsPromoterCMVAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-GPXC71S
Plasmid#191703PurposeTi plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701DepositorInsertchl-redox(ro)GFP2-GPXC71S
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPeroxidatic Cysteine 71 replaced with SerinePromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-2X snRP1
Plasmid#99688PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK)-12xHRE
Plasmid#172334Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGKAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP ratAPOBEC1 L173A/G227A
Plasmid#167169PurposeExpresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.DepositorArticleInsertAPOBEC1 (Apobec1 Rat)
UseTagsExpressionMammalianMutationchanged Leucine 173 to Alanine; changed Glycine 2…PromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER-G-Ca-FLITS-KDEL
Plasmid#191464PurposeGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
UseTagsER and KDELExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
4xmts-G-Ca-FLITS
Plasmid#191462PurposeGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
UseTags4xmtsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pminiT-MED6-Halo donor
Plasmid#197866PurposeDonor plasmid used for building MED6-Halo knock-in mESCsDepositorInsertMed6 (Med6 Mouse)
UseMouse TargetingTagsHalotagExpressionMutationPromoterNAAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS585P-myc
Plasmid#107498PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationS585PPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG484E-myc
Plasmid#107471PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationG484EPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS477F-myc
Plasmid#107470PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S477F loss of transport relative to wildtype, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationS477FPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65(150-261)-RTA(125-190)
Plasmid#99674PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-P65(151-261)-RTA(125-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTI425V-myc
Plasmid#107464PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425V conferring aproximately wildtype phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationI425VPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-1X snRP1
Plasmid#99687PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR-mOrange2
Plasmid#228608PurposeMammalian expression of mOrange2, for MuSIC barcode constructionDepositorInsertmOrange2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mBeRFP
Plasmid#228601PurposeMammalian expression of mBeRFP, for MuSIC barcode constructionDepositorInsertmBeRFP
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mTagBFP2
Plasmid#228596PurposeMammalian expression of mTagBFP2, for MuSIC barcode constructionDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-TJP1
Plasmid#227300PurposeDonor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)DepositorArticleInsertTJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-LSSmOrange
Plasmid#228600PurposeMammalian expression of LSSmOrange, for MuSIC barcode constructionDepositorInsertLSSmOrange
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only