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AKT3 gRNA (BRDN0001147841)

Plasmid

#76220

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT3 (AKT3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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JNK2 WT O/E (MAPK9)-pcw107-V5

Plasmid

#64617

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAPK9 (transcript variant JNK2-a2) (MAPK9 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

none

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223_MAP2K3_WT

Plasmid

#82140

Purpose

Gateway Donor vector containing MAP2K3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K3 (MAP2K3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT1-R121W

Plasmid

#116104

Purpose

Lentiviral expression of AKT1 R121W

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

R121W

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-MKNK2

Plasmid

#23735

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MKNK2 (MKNK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
myr-FLAG-MEK5-pcw107

Plasmid

#64620

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Lentiviral

Tags

Flag and myristoylated

Expression

Mutation

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-MKK6 (S207E,T211E)-pcw107

Plasmid

#64625

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K6 (MAP2K6 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

S207E,T211E

Promoter

PGK

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only
Flag_MmPERK_S503_N1114_pBabePuro

Plasmid

#58423

Purpose

retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

PERK (Eif2ak3 Mouse)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

Deleted amino acids M1 to Y502

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K1_WT

Plasmid

#82139

Purpose

Gateway Donor vector containing MAP2K1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

I310N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.L52R

Plasmid

#81604

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

L52R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_MAPK8_WT

Plasmid

#82229

Purpose

Gateway Donor vector containing MAPK8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK8 (MAPK8 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP2K1-P124S

Plasmid

#116428

Purpose

Lentiviral expression of MAP2K1 P124S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Tags

Expression

Mutation

P124S

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K1_WT

Plasmid

#81920

Purpose

Gateway Donor vector containing MAP2K1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

I310N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K4 gRNA (BRDN0001145410)

Plasmid

#76651

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K4 (MAP2K4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_MAP2K1_p.P124S

Plasmid

#81703

Purpose

Gateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P124S; I310N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K4 gRNA (BRDN0001148854)

Plasmid

#76652

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K4 (MAP2K4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-FLAG-MKK6

Plasmid

#21582

Depositor

John Kyriakis

Article

Yuasa et al J Biol Chem. 1998 Aug 28. 273(35):22681-

Insert

MKK6 (MAP2K6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pYX-N-Puro-mNeon-WNK3

Plasmid

#222674

Purpose

endogenously tag WNK3 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

WNK3 (WNK3 Human)

Use

Tags

mNeon

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-GRK6A/B [N142/3R]

Plasmid

#225381

Purpose

Mammalian Expression Plasmid of anti-GRK6A/B (Mouse) IgG2a R-mAb. Derived from hybridoma N142/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GRK6A/B (Mus musculus) recombinant (Mouse) monoclonal antibody. (Grk6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-PICK1 [L20/23R]

Plasmid

#220393

Purpose

Mammalian Expression Plasmid of anti-PICK1 scaffold protein (Human) IgG2a R-mAb. Derived from hybridoma L20/23.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-PICK1 scaffold protein (Homo sapiens) recombinant mouse monoclonal antibody. (PICK1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge