-
Plasmid#173129PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj3a (kcnj3a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj5
Plasmid#173132PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj5 (kcnj5 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj6
Plasmid#173133PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj6 (kcnj6 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFendoNA2S
Plasmid#174730PurposeExpresses GFP-endoNA2-S706A fusion protein for detection of polysialic acid. The endoNA2-S706A is a noncatalytic endosialidase that lacks the ability for releasing its C-terminal chaperone domain.DepositorInsertendoNA2-S706A
UseTagsExpressionBacterialMutationChanged Serine 706 to AlaninePromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnma1a
Plasmid#164951PurposeZebrafish kcnma1a gene CDSDepositorInsertkcnma1a (kcnma1a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L]
Plasmid#133330PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll FYW to L in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1507 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-S]
Plasmid#133331PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll VLIM to S in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F]
Plasmid#133332PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll VLIM to F in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2
Plasmid#124063PurposeFragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.DepositorInsertcytXIATL2
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1397 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]
Plasmid#118812PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationW385G in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1399 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W+G385W]
Plasmid#118813PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG368W and W385G in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1400 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295F]
Plasmid#118814PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG295F in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1393 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaRRM]
Plasmid#118805PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of RRM in full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1394 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaCTD]
Plasmid#118806PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of CTD in full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD]
Plasmid#118807PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of NTD in full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]
Plasmid#118808PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll F to S in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S]
Plasmid#118809PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll VLIM to S in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1405 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-F]
Plasmid#118810PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll VLIM to F in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_19kDa_EE
Plasmid#74951PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsGlu-Glu tag (EYMPME)ExpressionMammalianMutationdelta 1-127PromoterCMV, lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_23kDa_Myc
Plasmid#74949PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsHA Tag (YPYDVPDYA)ExpressionMammalianMutationdelta 1-99 M128APromoterCMV, Lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only