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Plasmid#133065PurposeRetroviral expression of mouse MD2 tagged with HADepositorInsertMD2 (Ly96 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sha2Cas9-HF
Plasmid#192142PurposeExpresses highly specific Sha2Cas9, and cloning backbone for sgRNADepositorInsertSha2Cas9-HF
UseAAVTagsExpressionMammalianMutationSha2Cas9-HF (R247A and N415A)PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-2xmCherry-EF(C)
Plasmid#61664PurposeTandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linkerDepositorInsertKIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
BTK-APEX2-P2A-mKate2
Plasmid#67653PurposeMammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertBTK-PH-APEX2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK3_001
Plasmid#123952PurposeEncodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK systemDepositorInsert3XNLS::CRY2PHR::p65
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-HA-GFP
Plasmid#120903PurposeExpresses Ptch1-B for function assaysDepositorInsertptch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-GFP11
Plasmid#61670PurposeNon-fluorescent, truncated kinesin-1 fused to split GFP linkerDepositorInsertKIF5C(1-560)-GFP11 (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK0_043
Plasmid#123943PurposeEncodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - A1 Insulator - HygroR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-2xmCitrine-GFP11
Plasmid#61667PurposeTandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linkerDepositorInsertKIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only