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pQE 80L Kan T7WT

Plasmid

#102790

Purpose

Expresses T7 RNA polymerase

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

T7 RNA polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

Plasmid

#61590

Purpose

Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

Depositor

David Komander

Article

Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

Insert

TRABID (ZRANB1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-244 and aa 698-708.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R248W

Plasmid

#16437

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R248W (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

R248W

Promoter

Available since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pThy1-Autobow

Plasmid

#45187

Depositor

Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pThy1-Autobow

Use

Linearized for transgenic mouse injection

Tags

Expression

Mutation

Promoter

Thy1

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pLJM1 Raptor GFP

Plasmid

#112744

Purpose

express Raptor-GFP

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Raptor-GFP (RPTOR Human)

Use

Lentiviral

Tags

eGFP

Expression

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASHΔPPPL

Plasmid

#129280

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

Depositor

Daniel Conway

Article

Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

the last 4 amino acids (PPPL) of the KASH domain …

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pIG-704_CD19-28z_CAR_tNGFR

Plasmid

#207486

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, CD19-28z_CAR (NGFR Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Article

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

ATG8

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin HL

Plasmid

#68128

Purpose

nesprin headless control

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

nesprin-2 (Syne2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

deleted actin-binding domain

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAB(EXPR-PYL-HDAC3-NEO)

Plasmid

#114394

Purpose

For PYL-HDAC3 expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC3 (HDAC3 Human)

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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