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pCEP4-Q842gp160

Plasmid

#100931

Purpose

Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q842.d12) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASHΔPPPL

Plasmid

#129280

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

Depositor

Daniel Conway

Article

Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

the last 4 amino acids (PPPL) of the KASH domain …

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_220

Plasmid

#91113

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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RNA motif plasmid cloning backbone

Plasmid

#107253

Purpose

RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

RNA motif plasmid cloning backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMPRA1

Plasmid

#49349

Purpose

Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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Lck-mTurquoise2

Plasmid

#98822

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Lck

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-NPC1

Plasmid

#132295

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

NPC1 (NPC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190077

Purpose

express human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2, TMPRSS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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DYRK1A_pCSdest

Plasmid

#53791

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

DYRK1A (DYRK1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcdna nesprin HL

Plasmid

#68128

Purpose

nesprin headless control

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

nesprin-2 (Syne2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

deleted actin-binding domain

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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