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pLKO5.sgRNA.EFS.PAC

Plasmid

#57825

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
PAC

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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p256

Plasmid

#186184

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 256

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p40PX-EGFP

Plasmid

#19010

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p40phox (NCF4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa1-148 (PX domains)

Promoter

CMV

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d1EGFP

Plasmid

#41942

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_rDA1h

Plasmid

#140557

Purpose

Expresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

Red fluorescent DA sensor GRAB_rDA1h (high affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SARS-CoV-2-S-RBD-sfGFP

Plasmid

#141184

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Has Service

Cloning Grade DNA

Insert

S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)

Use

Tags

Signal peptide from influenza HA and Superfolder …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28b-RfxCas13d-His

Plasmid

#141322

Purpose

Plasmid for bacterial expression and purification of RfxCas13d protein

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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p-dCas9-SSAP-MS2-BB_BbsI

Plasmid

#183826

Purpose

pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

U6-guideRNA-dCas9-T2A-EBFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBH

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a-SerRS-His

Plasmid

#124118

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Seryl-tRNA ligase (serS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

Plasmid

#138418

Purpose

CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Insert

dCas9

Use

Lentiviral

Tags

BioTAP-tag

Expression

Mutation

Promoter

EF1a

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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