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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

T5

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-dDIO hChR2(H134R)-EYFP

Plasmid

#55640

Purpose

Dre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Fon EYFP

Plasmid

#55650

Purpose

Cre-on/Flp-on EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Insert

eYFP with introns

Use

AAV; Cre on/flp on eyfp

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131004

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Expression

Mutation

Promoter

hSyn

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-hATG2A

Plasmid

#114462

Purpose

Expresses human ATG2A with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

Insert

human ATG2A (ATG2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

P656R (please see depositors comment below)

Promoter

CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130995

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Expression

Mutation

Promoter

hSyn

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon TVA-mCherry

Plasmid

#131779

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

TVA-mCherry

Use

AAV; Intrsect

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

nEF

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Flpo

Plasmid

#55634

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV1

Inserts

mCherry
Flpo

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a and Ef1a/IRES

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO5-pcDNA3.1-

Plasmid

#52509

Purpose

expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO5 (FBXO5 Human, FbxO5)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

Plasmid

#164827

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

miniCMV and pGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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HAP40 1-371

Plasmid

#124060

Purpose

Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HAP40 (F8A1 Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Expression

Mutation

Promoter

polyhedrin

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

Plasmid

#55643

Purpose

vCre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV; Vcre-dependent chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Medium_SynNotch_RHL144

Plasmid

#164828

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Medium Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Medium Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

miniCMV and pGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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K785R Smarca4-V5 IRES-puro

Plasmid

#107059

Purpose

Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

Depositor

Jerry Crabtree, Courtney Hodges

Article

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

IRES-puroR and V5

Expression

Mammalian

Mutation

K785R

Promoter

CAG

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBMN BFP-Parkin

Plasmid

#186221

Purpose

For expression of Parkin tagged with BFP

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

Parkin (PRKN Human)

Use

Retroviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_WT

Plasmid

#98653

Purpose

Expresses MBP-tagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMRXIB-mRuby3-CERT(PHD)

Plasmid

#221762

Purpose

Stable expression of mRuby3-CERT(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

CERT (CERT1 Human)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Mutation

amino acids 1–116

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m

Plasmid

#192275

Purpose

Encodes MmdCas12m (D485A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D485A

Promoter

PJ23108

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-XRCC1-EGFP-Hygro

Plasmid

#176062

Purpose

EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

X-ray repair cross complementing 1 (XRCC1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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