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Plasmid#52972Purposeinsect expression of SIK3 WTDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1208
Plasmid#44490DepositorInsertMinimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
UseGateway expression cloneTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmAGO1
Plasmid#22768DepositorInsertArgonaut-1, variant A (AGO1 Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
BSD-P2A-TagRFP-hSmad1-donor
Plasmid#203662PurposeDonor plasmid for CRISPR knock-inDepositorInsertsmad1 (SMAD1 Human)
UseTagsRFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST td tomato Rab5 WT
Plasmid#58446Purposeexpression of drosophila td tomato Rab5DepositorInsertRab5 (Rab5 Fly)
UseTagstd tomatoExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
NELFE-mOrange pHis-//
Plasmid#53303PurposeExpresses His-tagged NELF-E-mOrange fusion protein.DepositorInsertNELF-E protein (Nelf-E Fly)
UseTagsHis and mOrangeExpressionBacterialMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pUAST-Myc-dATG1
Plasmid#111801PurposeInsect Expression of dAtg1DepositorInsertdAtg1 (Atg1 Fly)
UseTagsMycExpressionInsectMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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ninaE-dsocs
Plasmid#26857DepositorInsertSlow Termination of Phototransduction (stops Fly)
UseTagsmycExpressionInsectMutationThis STOPS construct contains a mutated/truncated…PromoterAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159672PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertβTub-NLS-hSpCas9-T2A-GFP
UseTagsT2A-GFPExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
1161F
Plasmid#183138PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS
Plasmid#63170PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporterDepositorInsert"spaghetti monster GFP-OLLAS"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 mitochondrial matrix
Plasmid#172063PurposeUAS vector to express GFP1-10 in the mitochondrial matrixDepositorInsertGFP1-10 mitochondrial matrix
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-FUCCI-Luc
Plasmid#133364PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporterDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
UseTagsHA-tagged Clover-GemininExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-Squ:mCherry
Plasmid#20163DepositorInsertspaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pUASP dsRed Rab11 WT
Plasmid#44829DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pUASTLOTattB_mCherry::Nrv1::vhhGFP4
Plasmid#163926PurposeExpression of GrabFP-B-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only