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EGFP-Rab8b in pCSDEST2

Plasmid

#140881

Purpose

Expression of zebrafish GFP-tagged Rab8b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8b (rab8b Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-polySH33-EGFP

Plasmid

#175792

Purpose

Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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AICSDP-24:MYH10-mEGFP

Plasmid

#87428

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

MYH10 Homology Arms with mEGFP-linker (MYH10 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

May 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

Plasmid

#171795

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-eGFP

Plasmid

#179781

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

eGFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_AH

Plasmid

#148918

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SP146.gp91.eGFP

Plasmid

#30474

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 May 5. ():.

Insert

SP146/gp91/eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-m5HT6-EGFP

Plasmid

#200443

Purpose

Lentiviral expression of mouse m5HT6-EGFP

Depositor

Ken-Ichi Takemaru

Insert

5HT6-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1α

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct6

Plasmid

#28199

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa1-314 only

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pUC19-YAP-mEGFP

Plasmid

#166472

Purpose

HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Use

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLEGFP-K140R-STAT3

Plasmid

#71448

Purpose

Retroviral expression of K140R-STAT3. Please note that this plasmid contains K140R-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

K140R

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pGBK CUP1 EGFP

Plasmid

#170210

Purpose

Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFP

Depositor

Bruce Hamilton

Article

Hernandez et al bioRxiv

Insert

enhanced green fluorescent protein

Use

Tags

GAL4 DNA Binding Domain and Myc

Expression

Bacterial and Yeast

Mutation

Promoter

CUP1

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-RAX-WT

Plasmid

#194572

Purpose

Mammalian Expression of mEGFP-RAX WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RAX (RAX Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXF1-WT

Plasmid

#194568

Purpose

Mammalian Expression of mEGFP-FOXF1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXF1 (FOXF1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(R306C)

Plasmid

#110188

Purpose

Expression of mouse MeCP2 (R306C) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Arg 306 Cys

Promoter

CMV

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-cPHx2

Plasmid

#116854

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Use

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amino acids 169-329 (tandem dimer)

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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EHD2-T72A-mEGFP

Plasmid

#45934

Purpose

dominant-negative EHD2 mutant

Depositor

Ari Helenius

Article

Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

Insert

Eps-15 homology domain-containing protein2 (EHD2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

T 72 changed to A (T72A)

Promoter

CMV

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A-deltaCC

Plasmid

#166459

Purpose

Expresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltaCC (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-MPro-eGFP-2

Plasmid

#184824

Purpose

Expresses Mpro-eGFP in mammalian cells

Depositor

Wenshe Liu

Article

Cao et al ACS Cent Sci. 2022 Feb 23;8(2):192-204.

Insert

Mpro-eGFP conjugate (M SARS-CoV-2, jellyfish Aequorea Victoria)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1alfa

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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