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Plasmid#168121PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at C112/E115 within GNAI2PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only
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pFRT_TO_eGFP_TIA1
Plasmid#106094Purposegene expression of EGFP-TIA1DepositorInsertTIA1 (TIA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HyPer7-MEM
Plasmid#136465PurposeMammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsFarnselation tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
p53-TRE-mStrawberry reporter
Plasmid#158684PurposeThis plasmid is a p53 pathway reporter. It has two copies of wild-type p53 binding sites promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-q ONE-GO
Plasmid#189738PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-ARID1A
Plasmid#39311DepositorInsertARID1A (ARID1A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-EK_C122S_His5
Plasmid#49048PurposeExpresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coliDepositorInsertEnteropeptidase, peptidase domain (TMPRSS15 Bovine)
UseTags5xHisExpressionBacterialMutationP92R, E185D, C122SPromoterT7Available sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCold CL7-Cas12a
Plasmid#124891Purposeobtaining full Cas12a RNPDepositorInsertCas12a
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL-MS2-12X
Plasmid#27119DepositorInsertMS2 binding sites (12)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro PRMT5-CRISPRi
Plasmid#164637PurposelentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2DepositorInserttarget guide sequence PRMT5 inhibition (PRMT5 Human)
UseCRISPR and Lentiviral; InterferenceTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1(wt)
Plasmid#13719DepositorInsertEGFP-Rac1(wt) (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-145
Plasmid#118561PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3
Plasmid#108103PurposeLentiviral expression plasmid of human SIK3 cDNA with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRepair-MPP8_mAID-P2A-BFP
Plasmid#175036PurposeHomology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9DepositorInsertmAID-P2A-BFP
UseHdr donor templateTagsExpressionMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEC-red
Plasmid#196040PurposeAllows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate AssemblyDepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Grna cloningTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato
Plasmid#138610PurposeExpress TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backboneDepositorInsertsHyperactive AID
T7 RNA Polymerase
tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a mCherry
Plasmid#129431Purposeexpression of mCherry from EF1a promoterDepositorInsertmCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNTI825
Plasmid#216633PurposeCal-ID (V5) for AAV productionDepositorArticleInsertCal-ID
UseAAVTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FAH-rtTA3G
Plasmid#120309PurposeAn AAV vector that expresses rtTA3G under the c-fos promoterDepositorInsertrtTA3G
UseAAVTagsExpressionMutationPromoterc-fosAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-EGFP
Plasmid#86639PurposeExpresses mCherry-eGFP tandem (cytoplasmic)DepositorInsertmCherry-EGFP
UseTagsmCherry-EGFPExpressionMammalianMutationPromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143-ZmUbi-tRNA
Plasmid#158400PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-mIFNa2
Plasmid#135098PurposeProtein expression of mouse IFNa2 in mammalian cellsDepositorInsertmIFN-α2 (Ifna2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-IDH1-R132H
Plasmid#62907PurposeExpresses R132 mutant IDH1 in mammalian cellsDepositorInsertIDH1 (IDH1 Human)
UseTagsFlagExpressionMammalianMutationDeleted Arg132 (R132)PromoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-smGFP-Kv2.1
Plasmid#131709PurposeExpresses Kv2.1 in mammalian cellsDepositorInsertKCNB1 (KCNB1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-GST
Plasmid#215169PurposeEncodes the synthetic glutathionine S-transferase with N-terminal His tagDepositorInsertHis-GST
UseTagsHis-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
Plasmid#140003PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE NFKB-TA-LUC-UBC-dTomato-W
Plasmid#49335PurposepHAGE NFKB-TA-LUC-UBC-dTomato-WDepositorInsertsNFkB consensus binding sequence
TA
Luciferase
UBC
dTomato
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA3mt
Plasmid#140464PurposeRed fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertR-CEPIA3mt
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-IRES-RFP
Plasmid#35395DepositorInsertsUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCX-OKS-2A
Plasmid#19771PurposeNon-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2DepositorUseTagsExpressionMammalianMutationThree genes are connected with the foot-and-mouth…PromoterAvailable sinceJan. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3XHA-p53
Plasmid#196267PurposeMammalian expression of 3xHA tagged wild-type p53DepositorInsertp53 (Trp53 Mouse)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Luciferase
Plasmid#58792PurposeExpress FLAG-tagged Luciferase in mammalian cellsDepositorInsertfirefly Luciferase
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3 Flag p38 alpha (agf)
Plasmid#20352DepositorInsertpDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationReplace Thr 180 and Tyr 182 with Ala and Phe resp…PromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP
Plasmid#164038PurposeExpression of dCas9-14xGFP11 in mammalian cellsDepositorInsertdCas9-14xGFP11
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and H840A in Cas9PromoterdSV40Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
tetO-FOSL1
Plasmid#170687Purposedoxycycline-inducible overexpression of FOSL1DepositorInsertFOSL1 (FOSL1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAKlux2
Plasmid#14080DepositorInsertBacterial luciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChrimsonR-tdT
Plasmid#59171PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 Lnp-mCherry
Plasmid#86687PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorInsertLNPK (LNPK Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)
Plasmid#103870PurposeHigh specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.DepositorInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOP-flash
Plasmid#83467PurposeTEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cre-IRES-PuroR
Plasmid#30205DepositorInsertCre recombinase (cre Enterobacteria phage P1)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-RIGI-Apex- T2A-EGFP
Plasmid#167288PurposeLentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.DepositorInsertDDX58 (RIGI Human)
UseLentiviralTagsAPEX2 and EGFPExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Cm
Plasmid#174725PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLenti6-MK1-EHMT2-V5
Plasmid#31113DepositorInserteuchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationStop codon mutated to AlaPromoterAvailable sinceAug. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-wild type
Plasmid#61003PurposeExpresses wild type human PTPN14 in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCIP-AMPKa1_WT
Plasmid#79010PurposeFor mammalian expression of WT AMPK alpha1.DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseTagsExpressionMammalianMutationPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mito-LOV-Turbo-V5
Plasmid#199666Purposeexpresses LOV-Turbo in the mammalian mitochondria, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsCOX4I1 mito targeting sequence and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PC-GCaMP6f
Plasmid#73503PurposeGCaMP6f knock in construct into the AAVS1 locusDepositorInsertGCaMP6f
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFR_HCV_xb
Plasmid#11510DepositorInsertFirefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only