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TTR C10S S85C Y78F

Plasmid

#184746

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S85C, Y78F

Promoter

T7

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C A81T

Plasmid

#184745

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S85C, A81T

Promoter

T7

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C K80E

Plasmid

#184744

Purpose

bacterial expression of huamn TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S85C, K80E

Promoter

T7

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0033

Plasmid

#185624

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-CTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-2

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0032

Plasmid

#185623

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-1

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEGPN

Plasmid

#187482

Purpose

Expression vector - GFP

Depositor

Lewis Cantley

Article

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C I84S

Plasmid

#184748

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S85C, I84S

Promoter

T7

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF1-L1-dSpRY

Plasmid

#177681

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dCas9-SpRY

Use

CRISPR; Gateway compatible foki-dcas9-spry entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF1-L2-dSpRY

Plasmid

#177682

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dCas9-SpRY

Use

CRISPR; Gateway compatible foki-dcas9-spry entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L2-dMb2Cas12a

Plasmid

#177684

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dMb2Cas12a

Use

CRISPR; Gateway compatible foki-dmb2cas12a entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBM13g-sd4U

Plasmid

#179689

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N (S1330I + A1341V + N1342S + E1370K + T1380I)

Use

Tags

Expression

Bacterial

Mutation

S1330I A1341V N1342S E1370K T1380I

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0058

Plasmid

#177022

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol 8-oxidase; geraniol-10-hydroxylase; CYP76B6

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0905

Plasmid

#177068

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of gal4AD-phiC31 driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

gal4AD-phiC31

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPKK0CM0480

Plasmid

#177037

Purpose

Level 0 promoter binding site part for MoClo assembly, nonstandard overlaps, CCAT-TACT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

2x opattB binding site plus minimal CaMV 35S promoter plus partial TMV 'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0705

Plasmid

#177035

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

phiC31 integrase plus SV40 nuclear localization sequence (PKKKRKV)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0812

Plasmid

#177034

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

gal4 activation domain plus GGSGGGGSGG linker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0910

Plasmid

#177033

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

loganic acid O-methyltransferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0766

Plasmid

#177032

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

strictosidine synthase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0764

Plasmid

#177030

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

secologanin synthase; CYP72C

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0763

Plasmid

#177029

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

loganic acid O-methyltransferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge