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Showing: 2121 - 2140 of 3194 results

  1. Linked mRuby3 VLP

    Plasmid
    #182430
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Li Chen Cheah et al BioRxiv, 2022
    Inserts
    VP2C-GFP-mRuby3
    deltaVP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Availability
    Academic Institutions and Nonprofits only
    182430-plasmid-map-sequence-id-360112

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  2. Coexpressed mRuby3 VLP

    Plasmid
    #182431
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Li Chen Cheah et al BioRxiv, 2022
    Inserts
    VP2C-GFP
    deltaVP1
    VP2C-mRuby3
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Availability
    Academic Institutions and Nonprofits only
    182431-plasmid-map-sequence-id-360113

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  3. Linked Free NES (M)

    Plasmid
    #182436
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Li Chen Cheah et al BioRxiv, 2022
    Insert
    FPPS(M)-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Availability
    Academic Institutions and Nonprofits only
    182436-plasmid-map-sequence-id-360120

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  4. Coexpressed Free NES (M)

    Plasmid
    #182437
    Purpose
    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Li Chen Cheah et al BioRxiv, 2022
    Inserts
    FPPS(M)
    NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Availability
    Academic Institutions and Nonprofits only
    182437-plasmid-map-sequence-id-360121

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  5. pPolI_SARS-CoV-2_NLuc

    Plasmid
    #183953
    Purpose
    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter
    Depositor
    AJ te Velthuis
    Article
    Vial et al bioRxiv 2021.06.28.450211
    Insert
    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mammalian Pol I
    Availability
    Academic Institutions and Nonprofits only
    183953-plasmid-map-sequence-id-365335

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  12. pX330-PITCh-ACTB

    Plasmid
    #207748
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    sgRNA Targeting N-terminus of ACTB (ACTB Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    207748-plasmid-map-sequence-id-414798

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  13. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid
    #207760
    Purpose
    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    207760-plasmid-map-sequence-id-413789

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  14. pX330-PITCh-LMNB1

    Plasmid
    #207770
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    207770-plasmid-map-sequence-id-416199

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  15. qTAG-C-Solo-mScarlet-H3C2

    Plasmid
    #207781
    Purpose
    Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    H3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    207781-plasmid-map-sequence-id-416187

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  16. qTAG-C-mNeon-Blast-H3C2

    Plasmid
    #207782
    Purpose
    Donor template for mNeon-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    H3C2 Homology Arms flanking a mNeon-Blast Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    207782-plasmid-map-sequence-id-416186

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  17. qTAG-C-moxGFP-Puro-H3C2

    Plasmid
    #207783
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    207783-plasmid-map-sequence-id-416185

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  18. qTAG-C-miRFP670nano3-Blast-H3C2

    Plasmid
    #207784
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    207784-plasmid-map-sequence-id-416184

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Showing: 2121 - 2140 of 3194 results