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Plasmid#166102PurposeThis plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.DepositorArticleInsertIDH2 sgRNA #7 (IDH2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH1 #4
Plasmid#166101PurposeThis plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.DepositorArticleInsertIDH1 sgRNA #4 (IDH1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgCTRL-3
Plasmid#83936PurposeLentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.DepositorInsertsgCTRL-3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shNuf2
Plasmid#160961PurposeNuf2 shRNA in pMKO.1 retroviral vectorDepositorInsertNuf2 shRNA (Nuf2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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px335-sgRNA-EF(Cas9n)-Ddx42-R
Plasmid#122344PurposeExpresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Ddx42
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Npm1-L
Plasmid#122332PurposeExpresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Npm1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)-Cdt1
Plasmid#122342PurposeExpresses sgRNA targeting mouse Cdt1 and eSpCas9 in mammalian cellsDepositorInsertsgRNA for mouse Cdt1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Dnmt3l-L
Plasmid#122321PurposeExpresses sgRNA targeting mouse Dnmt3l and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Dnmt3l
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Dnmt3l-R
Plasmid#122322PurposeExpresses sgRNA targeting mouse Dnmt3l and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Dnmt3l
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Jade1-L
Plasmid#122325PurposeExpresses sgRNA targeting mouse Jade1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Jade1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Ssrp1-L
Plasmid#122315PurposeExpresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Ssrp1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
MASTL F4.1 gRNA
Plasmid#90753Purpose3rd generation lentiviral gRNA plasmid targeting human MASTLDepositorInsertMASTL (Guide Designation F4.1)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVsc.U6-sgAspa.CMV/CB-EGFP
Plasmid#121510PurposeExpresses sgRNA targeting mouse Aspa intron 2 (sgAspa).DepositorInsertsgAspa
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNAs[bTub+Tra].1046D
Plasmid#112693Purposeexpress two gRNA targeting bTub & Tra under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorInsertLacI-LAP-HEC1 (NDC80 Human)
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-shLARP7
Plasmid#113537PurposeRetroviral vector designed to knock down LARP7.DepositorInsertshLARP7 (LARP7 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR sgAMDHD1
Plasmid#102316Purposegenetic depletion of AMDHD1DepositorInsertsgAMDHD1 (AMDHD1 Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
plko-tet-on puromycin-shMTH1
Plasmid#110918PurposeTo knock down MTH1 (Mut T Homolog 1) after addition of doxyclineDepositorInsertMTH1 (NUDT1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -