Addgene

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pC-KIT3

Plasmid

#118985

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

K2-mutSP-K1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT5

Plasmid

#118987

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

mutK2-mutSP-K1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT7

Plasmid

#118989

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

K2-mutSP-mutK1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-KD

Plasmid

#114066

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-KD (TTK Human)

Use

Tags

GST

Expression

Insect

Mutation

Mps1 KD (D664A)

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAM5080

Plasmid

#85106

Purpose

Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

YFP-KaiC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Use

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

Plasmid

#91933

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc

Use

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _1-480

Plasmid

#86817

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)

Plasmid

#79035

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX4T-Mst2 SARAH

Plasmid

#84298

Purpose

Expresses Mst2 SARAH domain for interaction and spectroscopy studies.

Depositor

Jonathan Chernoff

Article

Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

Insert

Mst2 (STK3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

L443W

Promoter

tac

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
TU#1805_CRISPR_unc-73_exon2

Plasmid

#82359

Purpose

to create unc-73B null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

Plasmid

#79380

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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hs-FL-Kek1-myc2

Plasmid

#41170

Depositor

Norbert Perrimon

Article

Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

Insert

Kek1 (kek1 Fly)

Use

Tags

2X myc

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00684

Plasmid

#39728

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12630 (tio Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pIS53 SERP1 3'UTR mut

Plasmid

#14500

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

SERP1 3'UTR mutant (SERP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
3575 pSFFV-neo Bad G148A

Plasmid

#8794

Depositor

Stanley Korsmeyer

Article

Zha et al J Biol Chem 1997 Sep 26;272(39):24101-4.

Insert

Bad (Bad Mouse)

Use

Tags

Expression

Mammalian

Mutation

G148A

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
3576 pSFFV-neo Bad R149A

Plasmid

#8795

Depositor

Stanley Korsmeyer

Article

Zha et al J Biol Chem 1997 Sep 26;272(39):24101-4.

Insert

Bad (Bad Mouse)

Use

Tags

Expression

Mammalian

Mutation

R149A

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
3577 pSFFV-neo Bad L151A

Plasmid

#8796

Depositor

Stanley Korsmeyer

Article

Zha et al J Biol Chem 1997 Sep 26;272(39):24101-4.

Insert

Bad (Bad Mouse)

Use

Tags

Expression

Mammalian

Mutation

L151A

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

Plasmid

#79379

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

Plasmid

#79382

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge