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Plasmid#158458PurposeConstitutive lentiviral expression of human TMPRSS2.DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCHA-VRC01-scFv
Plasmid#44874DepositorInsertVRC01 scFv
UseTagsHA and MycExpressionYeastMutationPromoterGAL1Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA E2F1
Plasmid#24225PurposeMammalian expression of human E2F1 with HA tagDepositorInsertE2F1 (E2F1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pREDawn-AmpR-MCS
Plasmid#188978PurposeFor red light-induced bacterial expression of gene of interest (AmpR)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1
Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PE2max-BSD
Plasmid#191102PurposeLentiviral expression plasmid of PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseCRISPR and LentiviralTagsExpressionMammalianMutationChanged R221K, N394K, H840A from SpCas9PromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpliceExpress
Plasmid#32485DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pZ8-Ptac
Plasmid#74064PurposeIPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNX32-Dest
Plasmid#105083PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneUseTagsNubGExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAP-delta10-cHIS
Plasmid#21716DepositorInsertpolyA polymerase (PAP1 Budding Yeast)
UseTagsHISExpressionBacterialMutation30 amino acids off the c terminusPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_ELAVL1
Plasmid#106104PurposeInducible gene expression of FlagHA ELAVL1DepositorInsertELAVL1 (ELAVL1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
SupD v2
Plasmid#198598PurposePlasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.DepositorInsertSupD
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT018-LAT_WT-HaloTag-3x FLAG
Plasmid#202502PurposeExpress WT-LATDepositorInsertLAT (LAT Human)
UseTagsHaloTag-3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (GFP)
Plasmid#80924PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
DS-SPcas
Plasmid#48645PurposeBacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
tracrRNA precursor
UseCRISPRTagsExpressionMutationPromoterproC and tracrRNA promoterAvailable sinceDec. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T286D)
Plasmid#127391PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 286 to Aspartic acidPromoterpCAGAvailable sinceJan. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS118 His14-Avi-28xGS-anti ALFA tag nanobody
Plasmid#199371PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-ALFA nanobody
UseTags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-BirA-ER
Plasmid#20856DepositorInsertBirA-ER
UseTagsExpressionMammalianMutationC-terminal ER targeting sequence (KDEL)PromoterAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40
Plasmid#100834PurposeAAV expression of ultrasensitive protein calcium sensor from CamKII promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCamKIIAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK3 wt
Plasmid#80873Purposemammalian expression of ALK3 WTDepositorInsertALK3 (BMPR1A Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-Expotin 1
Plasmid#42047DepositorInsertExportin-1 (XPO1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-N108Q
Plasmid#194157PurposeMammalian expression of ABE8e-N108QDepositorInsertTadA-8e (N108Q)-nSpCas9
UseCRISPRTags6*HisExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab5CA(Q79L)
Plasmid#35138DepositorInsertRab5a (RAB5A Human)
UseTagsmCherryExpressionMammalianMutationConstitutively Active- Q79LPromoterCMVAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-EKAR4
Plasmid#205513PurposeGenetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.DepositorInsertendo-EKAR4
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gα13*-BERKY3
Plasmid#158218PurposeBRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7 RNA POL
Plasmid#138352PurposeExpressing T7 RNA Polymerase in mammalian cellsDepositorInsertT7 RNA POL
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_3xFlagEzh2 WT
Plasmid#173717PurposeWT EZH2 expression plasmid with 3XFLAG tagDepositorInsertEZH2 (EZH2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hMALAT1
Plasmid#118580Purposeto overexpress human MALAT1 in mammalian cellsDepositorInsertMALAT1 (MALAT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible Luciferase reporter
Plasmid#64127PurposePhotoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.DepositorInsertluciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterTetOx13-CMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_WiChR_TS_mScarlet_ER
Plasmid#205997PurposeHigh efficient, K+ selective channelrhodopsin for the inhibition of neuronsDepositorInsertWobblia Inhibitory Channelrhodopsin
UseAAVTagsTS, mScarlet, ERExpressionMutationPromoterhSynAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6f.WPRE.SV40
Plasmid#100835PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696Purposeallows co-translational incorporation of a fluorescent AA directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJFRC19-13XLexAop2-IVS-myr::GFP
Plasmid#26224DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-Cerulean(1-10)
Plasmid#165144PurposeExpresses Cerulean(1-10) in mammalian cellsDepositorInsertCerulean(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorInsertssSpCas9
gRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAKTaq
Plasmid#25712PurposeBacterial expression of Taq polymeraseDepositorInsertTaq polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Rootletin (Nigg pFL2(CW499))
Plasmid#41166DepositorInsertRootletin (CROCC Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV- δLight
Plasmid#201224PurposeExpress deltaLight biosensor in mammalian cellsDepositorInsertδLight1.3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-SIRT6
Plasmid#102325PurposeHuman SIRT6 for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE
Plasmid#162381PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationA25G F286YPromoterCAGAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE
Plasmid#125256Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selectionDepositorInserthChR2(H134R)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1a-forwardAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_r5-HT1.0
Plasmid#208719PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neuronsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-APOBEC1-YTH
Plasmid#131636PurposeExpresses APOBEC1 fused to the YTH domainDepositorInsertAPOBEC1 fused to YTH domain (YTHDF2 Human, Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.cTNT.PI.eGFP.WPRE.rBG
Plasmid#105543PurposeAAV expression of EGFP from cTNT promoterDepositorHas ServiceAAV9InsertcTNT-PI-EGFP
UseAAVTagsExpressionMammalianMutationPromotercTNTAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-hAXL
Plasmid#124797PurposeTet-inducible AXL expression in mammalian cellsDepositorInsertAXL (AXL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy55
Plasmid#122007PurposeMammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis10-SumoStar (Life Sensors) and Multi2: dependi…ExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2/103Q
Plasmid#1385DepositorInserthuntingtin (103Q) (HTT Human)
UseTagsEGFP and FLAGExpressionYeastMutationExon1 sequences containing the first 17 amino aci…PromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
p{CFD4-3xP3::DsRed}
Plasmid#86864PurposeExpresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
ITGAV-bio-His
Plasmid#51919PurposeExpresses full-length Integrin alpha-V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertITGAV (ITGAV Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only