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Showing: 5301 - 5350 of 156661 results

  3. pSHDY

    Plasmid
    #137661
    Purpose
    Conjugative vector with streamlined cloning capabilities. Contains two cloning sites for modular insertion of genetic parts.
    Depositor
    Ilka Axmann
    Article
    Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137661-plasmid-map-sequence-id-271675

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  4. pIRES LifeAct-GFP puro3

    Plasmid
    #64357
    Purpose
    Bicistronic expression of LifeAct-GFP fusion and puro3 resistance gene
    Depositor
    Matthieu Piel
    Insert
    LifeAct-GFP
    Use
    Tags
    IRES-Puro
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pBiFC-VC155

    Plasmid
    #22011
    Purpose
    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays
    Depositor
    Chang-Deng Hu
    Article
    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.
    Insert
    VC155
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Alanine 206 to Lysine
    Promoter
    Available since
    Sept. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    22011-plasmid-map-sequence-id-354492

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  9. pBS L30 mRuby3-Rab6b

    Plasmid
    #166427
    Purpose
    Rab5C fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Insert
    Rab6B
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    L30 weak promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166427-plasmid-map-sequence-id-333504

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  15. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid
    #128386
    Purpose
    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Human, Synthetic)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    C-terminal luc2 fragment and N-terminal luc2 frag…
    Expression
    Mammalian
    Mutation
    N terminal carbohydrate recognition domain fused …
    Promoter
    EF1A and IRES
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    128386-plasmid-map-sequence-id-263327

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  26. p-dCas9-G9a-Hygro

    Plasmid
    #104408
    Purpose
    transient expression of dCas9-G9a fusion protein
    Depositor
    Stephan Beck
    Insert
    G9a (EHMT2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104408-plasmid-map-sequence-id-204469

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  38. pSBtet-RH

    Plasmid
    #60500
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60500-plasmid-map-sequence-id-97547

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  40. mEmerald-TOMM20-N-10

    Plasmid
    #54282
    Purpose
    Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54282-plasmid-map-sequence-id-257386

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  41. pExp-TF

    Plasmid
    #112575
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-TriggerFactor and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112575-plasmid-map-sequence-id-217211

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  47. JMJD2A

    Plasmid
    #38846
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JMJD2A (Kdm4a Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. pSBtet-BP

    Plasmid
    #60496
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60496-plasmid-map-sequence-id-97551

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  50. TFORF1759

    Plasmid
    #143845
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ALYREF (ALYREF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143845-plasmid-map-sequence-id-396272

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Showing: 5301 - 5350 of 156661 results