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pSL0373 (His-MBP-TniQ)

Plasmid

#130638

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ST-SBP-EGFP_puromycin

Plasmid

#65270

Purpose

ST reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ManII-SBP-tagBFP

Plasmid

#65254

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pDIV490

Plasmid

#177698

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Mutation

Promoter

RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSL0721 (pDonor_L1-105)

Plasmid

#130645

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short L)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0715 (pDonor_R1-57)

Plasmid

#130644

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short R)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ST-SBP-mCherry_puromycin

Plasmid

#65271

Purpose

ST reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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SMT205

Plasmid

#134817

Purpose

SMT201 with mutated RBS for DHFR, in vivo assays

Depositor

Tanja Kortemme

Article

Thompson et al Elife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Use

Tags

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7 and TET

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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His-MBP-NRBF2 (1-159)

Plasmid

#99331

Purpose

Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Use

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

AA1-159 are present

Promoter

T7

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pSD103D

Plasmid

#218134

Purpose

For competition during selection with hybrid circuit. Expresses MBP fused to evolved degron SD55

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD55

Use

Tags

MBP

Expression

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pNK6031

Plasmid

#219739

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter CmYLCV

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

Cestrum yellow leaf curling virus (CmYLCV) promoter

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-MGT4-d2EGFP_CMV-mCherry

Plasmid

#213764

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

MGT#4-d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-CLGT3-d2EGFP_CMV-mCherry

Plasmid

#213766

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

CLGT#3-d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP

Plasmid

#210509

Purpose

expresses PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP component in rat hippocampal neuron cells

Depositor

Dongmin Lee

Inserts

hSyn promoter (RIC8B )
PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG101

Plasmid

#189590

Purpose

To make ATG101/ATG13 HORMA complex

Depositor

James Hurley

Article

Shi et al J Cell Biol. 2020 Jul 6;219(7):e20191104

Insert

ATG101 (ATG101 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1936

Plasmid

#195692

Purpose

Complementation of MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1935

Plasmid

#195693

Purpose

Complementation of MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1953

Plasmid

#195694

Purpose

Complementation of MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1933

Plasmid

#195695

Purpose

Complementation of MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1934

Plasmid

#195696

Purpose

Complementation of MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge