hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP
(Plasmid #210509)

• Purpose: expresses PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP component in rat hippocampal neuron cells
• Depositing Lab: Dongmin Lee
• Publication: A single-component, light-assisted uncaging switch for endoproteolytic release (unpublished)

Backbone

• Vector backbone: pSyc181 P2A
• Total vector size (bp): 6900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: hSyn promoter
• Entrez Gene: RIC8B (a.k.a. RIC8, hSyn)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: attacgccagtcgactAGTGCAAGTGGGTTTTAG
• 3′ sequencing primer: aagcttCTGCGCTCTCAGGCAC

Gene/Insert 2

• Gene/Insert name: PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP
• Insert Size (bp): 3405
• Promoter: hSyn
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: AAA TGG GCG GTA GGC GTG
• 3′ sequencing primer: GAA ACA GCT ATG ACC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-1628455/v1 for preprint.