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Showing: 2101 - 2120 of 2728 results

  4. SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)

    Plasmid
    #149278
    Purpose
    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs
    Depositor
    Savithramma Dinesh-Kumar
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    Modified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence
    Use
    T-dna vector
    Tags
    Expression
    Mutation
    T2318C (DNA) in PEBV coat protein sequence
    Promoter
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    149278-plasmid-map-sequence-id-300518

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  5. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid
    #48227
    Purpose
    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    Use
    CRISPR
    Tags
    2A-neo, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    48227-plasmid-map-sequence-id-69517

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  8. pSLQ10871

    Plasmid
    #183962
    Purpose
    U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    dCas12a(wildtype)
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG and U6
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183962-plasmid-map-sequence-id-363117

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  9. pYPQ-dzCas9-NG-Act3.0

    Plasmid
    #158415
    Purpose
    CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158415-plasmid-map-sequence-id-331006

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  12. LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

    Plasmid
    #170254
    Purpose
    Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    STAT3 (STAT3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    A662C, N664C, V667L
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170254-plasmid-map-sequence-id-334374

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  15. pYPQ-dAaCas12b-Act3.0

    Plasmid
    #158413
    Purpose
    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158413-plasmid-map-sequence-id-337077

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  19. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

    Plasmid
    #170220
    Purpose
    Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    CDK1 (CDK1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    T14A, Y15F
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170220-plasmid-map-sequence-id-334386

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Showing: 2101 - 2120 of 2728 results