-
-
pENTR-L1R5-floxHygpA
Plasmid#139559Purposefloxed Hygromycin resistant gene with stop codon franking to pA sequeneces between attL1-attR5 on Gateway entry vectorDepositorInsertHygromycin resistant gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-CDK13_IDG-K
Plasmid#135276PurposeGateway destination clone of CDK13 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK13 (CDK13 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-7.4kb
Plasmid#58891Purposeprovides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
UseGateway 5' entry cloneTagsExpressionMutationmutated single nucleotide (T-->C, 1874th nucle…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-CDK13_IDG-K
Plasmid#135268PurposeGateway destination clone of CDK13 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-CDK13 (CDK13 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-mnx1
Plasmid#82402PurposeGateway-compatible zebrafish mnx1 promoterDepositorInsertmnx1 promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-ALPK2-V5-miniTurbo_IDG-K
Plasmid#198009PurposeGateway destination clone of ALPK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertALPK2-V5-miniTurbo (ALPK2 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-TurboID-V5-GSK3B_IDG-K
Plasmid#158164PurposeGateway destination clone of GSK3B (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTurboID-V5-GSK3B (GSK3B Human)
UseLentiviral; Gateway destinationTagsTurboID-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-hPGK
Plasmid#82579Purpose5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expressionDepositorInsertHuman PGK promoter
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBSDONR P1-P4
Plasmid#71744Purposeampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloningDepositorTypeEmpty backboneUseGateway entry vector for use in multisite gateway…TagsExpressionMutationPromoterAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-gfap
Plasmid#82401PurposeGateway-compatible zebrafish gfap promoterDepositorInsertgfap promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-CDK12_IDG-K
Plasmid#135281PurposeGateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK12 (CDK12 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#82400PurposeGateway-compatible zebrafish elavl3 promoterDepositorInsertelavl3 promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK14_IDG-K
Plasmid#158172PurposeGateway destination clone of CDK14 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK14 (CDK14 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
469_pME_iCre
Plasmid#171792PurposepME-Vector with codon improved iCre sequence. Tol2-kit compatible.DepositorInsertcodon-optimized iCre sequence
UseTagsExpressionMutationPromoterNoneAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b
Plasmid#83129PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions)DepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-Tandem-mCherry-EGFP
Plasmid#123216PurposeN-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a
Plasmid#83166PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)DepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-DYRK1B_IDG-K
Plasmid#158183PurposeGateway destination clone of DYRK1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-DYRK1B (DYRK1B Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-P2A-mKate2 no-pA
Plasmid#80828PurposeP2A followed by mKate2 without pADepositorInsertp3E-P2A-mKate2 no pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only
Holiday Schedule: Addgene will be closing at 1:00 pm EST on November 27th and remain closed through the 29th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
How are we doing? Share feedback for a chance to win $50
Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more
Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more
