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Plasmid#225481PurposeExpress gRNA for FXR1DepositorInsertgRNA for editing FXR1-N202S (FXR1 Human)
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-Tudor-R rich
Plasmid#225478PurposeExpress mEGFP-GAPDH-Tudor-R fusionDepositorInsertGAPDH fused with Tudor and R-rich regions of TDRD3 (GAPDH Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-PODXL S481D
Plasmid#195224PurposeMammalian expression vector containing GFP tagged Podocalyxin. PKC phosphomimetic mutant.DepositorInsertPODXL (PODXL Rabbit)
UseTagsExpressionMammalianMutationS481DPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB143
Plasmid#222214PurposeFor recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tagDepositorInsertvanB(A24P)
UseTagsN-terminal, thrombin-cleavable 6x His tagExpressionBacterialMutationChanged Alanine 24 to ProlinePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN95
Plasmid#222220PurposeFor recombinant expression of vanA (PP_3736 from Pseudomonas putida) with a C-terminal His tagDepositorInsertvanA putida (PP_RS19450 Pseudomonas putida KT2440)
UseTags6x HisExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB109
Plasmid#222208PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tagDepositorInsertfghA (K10N)
UseTags6x HisExpressionBacterialMutationChanged Lys10 to AsnPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB110
Plasmid#222209PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tagDepositorInsertfghA (G258D)
UseTags6x HisExpressionBacterialMutationChanged Gly258 to AspartatePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB111
Plasmid#222210PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tagDepositorInsertfghA(G76V)
UseTags6x HisExpressionBacterialMutationChanged glycine 76 to valinePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB112
Plasmid#222211PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tagDepositorInsertfghA(S11R)
UseTags6x HisExpressionBacterialMutationChanged Serine 11 to ArgininePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB113
Plasmid#222212PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tagDepositorInsertfghA(W15C)
UseTags6x HisExpressionBacterialMutationChanged Tryptophan 15 to CysteinePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS exon37a pCAGGS
Plasmid#206086Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37aDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
EYFP Cav2.2 pRK5
Plasmid#206099Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal EYFP tag and an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA pCAGGS
Plasmid#206054Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA pCAGGS
Plasmid#206052Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA pcDNA3
Plasmid#206051Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA pcDNA3
Plasmid#206053Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_B1ChR2_TS_mScarlet_ER
Plasmid#195191PurposeHighly K+ selective channelrhodopsinDepositorInsertBilabrum sp. K+ selective channelrhodopsin 2
UseTagsER-export sequence, Kir2.1 trafficking motif, and…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-FLAG-Rab11
Plasmid#194336PurposeExpresses FLAG tagged Rab11 in mammalian cells and zebrafish embryosDepositorInsertRab11 (RAB11A Rabbit)
UseTagsFLAGExpressionMammalianMutationPromoterCMV IE94Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-DrCavin1b
Plasmid#194292PurposeMultisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJDepositorInsertcavin1b (cavin1b Zebrafish)
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7gRNA-smyhc1_977
Plasmid#140867PurposesgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1).DepositorInsertzebrafish smyhc1_977 sgRNA for in vitro transcription (smyhc1 Zebrafish, Synthetic)
UseIn vitro transcription of sgrnasTagsExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only