Addgene

Showing: 2101 - 2120 of 148384 results

Results per page:

Sort results by:

CMV-mEGFP(A206K)-paCaMKII

Plasmid

#165438

Purpose

Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII

Use

Tags

mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-mC/EBPb

Plasmid

#49198

Purpose

expression of mouse C/EBPbeta in mammalian cells

Depositor

Jed Friedman

Insert

C/EBPbeta (Cebpb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMMP-dnPERK-IRES-eGFP

Plasmid

#36954

Depositor

Bill Sugden

Article

Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

Insert

dnPERK (EIF2AK3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

deletion of aa592-1061 to create dominant negative

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Flag Rac2

Plasmid

#12192

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
HDAC1 Flag

Plasmid

#13820

Purpose

Mammalian expression of human histone deacetylase 1 with flag tag

Depositor

Eric Verdin

Article

Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

Insert

HDAC1 (HDAC1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN-HA-NAP1

Plasmid

#208868

Purpose

For mammalian expression of NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (NAP1L1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2-HR1b-GFP-L (B3-B4)

Plasmid

#211853

Purpose

E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Tags

GFP

Expression

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX209-neo-active WRN

Plasmid

#125788

Purpose

constitutive, ectopic expression of human active WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-active WRN (WRN Human)

Use

Tags

5x myc

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-hMLH1dn for IVT

Plasmid

#178114

Purpose

Template for in vitro transcription of human MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized) (MLH1 Human)

Use

Template for in vitro transcription

Tags

Expression

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsEDC4_S

Plasmid

#147455

Purpose

Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsEDC4 (EDC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hCtRNA_FT

Plasmid

#186715

Purpose

Fragment template for DAP array

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

gRNA scaffold and hCtRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hCtRNA

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-mCherry

Plasmid

#180646

Purpose

Lentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EZ-Tet-pLKO-Puro

Plasmid

#85966

Purpose

Tet/Dox inducible shRNA lentivirus with puromycin selection

Depositor

Cindy Miranti

Article

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

H1 / TetO

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FAB(ER-LD)

Plasmid

#214424

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Use

Tags

CFAST-Halo and RspAN-FRB-V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER20_mStrawberry_Atg4BC74A

Plasmid

#161735

Purpose

Doxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagy

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

mStrawberry

Expression

Mammalian

Mutation

220-222 TGC (Cys) is changed to GCA (Ala)

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
2xP53_RE::NLuc

Plasmid

#124533

Purpose

p53 NLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

P53 NLuc reporter

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
DPYD-FLAG

Plasmid

#61616

Purpose

The human DPYD was used to study the effect of DPYD expression on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

Dihydropyrimidine dehydrogenase (DPYD Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR SFFVp BFP-Erk2

Plasmid

#50848

Purpose

Fluorescent reporter for Erk nuclear translocation

Depositor

Wendell Lim

Article

Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

TagBFP

Expression

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
myc EDC4 pCMVSport6

Plasmid

#86855

Purpose

mammalian expression of EDC4

Depositor

Sarah Slavoff

Article

D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

Insert

EDC4 (EDC4 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-tetO-Gata3

Plasmid

#70270

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Gata3

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

GATA binding protein 3 (Gata3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge