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Plasmid#183885PurposepX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.DepositorInsertACTB sgRNA spacer (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE
Plasmid#196677PurposeAAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).DepositorInsertPKIα-P2A-mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pODN-AAVS1-mR2-CAAX
Plasmid#183871PurposeRepair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.DepositorInsertAAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
UseCRISPR; Donor templateTagsmRuby2-CAAXExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterCMVAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHS1543
Plasmid#205963PurposeNZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)DepositorInsertDinG HNH Cascade with His14-tagged Csf3
UseTags14xHis on Csf3ExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Ace-mNeon2-Kv2.1PR
Plasmid#195522PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterSynapsinAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1929
Plasmid#205968PurposeJAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1DepositorInsertCas8-HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-PA-mCherry
Plasmid#138251PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-PAmCherry
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-OSBP
Plasmid#67664PurposeMammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-OSBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM50
Plasmid#123656PurposeEncodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cellsDepositorInsertAedes aegypti UbL40 promoter
UseLuciferaseTagsExpressionInsectMutationPromoterUbL40 promoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHS1068
Plasmid#205962PurposeNZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promotersDepositorInsertDinG HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only