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Plasmid#193763PurposeDoxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1-mCherry (CDT1 Human)
UseLentiviralTagsWTExpressionMutationhuman Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cdt1ΔCy-mCherry-Puro
Plasmid#193764PurposeDoxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1ΔCy-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationCy motif (a.a. 68-70) mutated to alanine, a.a. 11…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1-mCherry-Puro
Plasmid#193766PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1Δ499-546-HA-Puro
Plasmid#193768PurposeDoxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1Δ499-546 (CDT1 Human)
UseLentiviralTagsHAExpressionMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
NPY/Neuropeptide Y scFv [L115/13]
Plasmid#190505PurposeMammalian Expression of NPY/Neuropeptide Y scFV. Derived from hybridoma L115/13.DepositorInsertNPY/Neuropeptide Y (Homo sapiens) recombinant scFV (NPY Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-Neuroligin-2 [L107/90R]
Plasmid#188169PurposeMammalian Expression Plasmid of anti-Neuroligin-2 (Mouse). Derived from hybridoma L107/90DepositorInsertanti-Neuroligin-2 (Mus musculus) recombinant mouse monoclonal antibody (Nlgn2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hCHAT)-PGKpuro2ABFP-W
Plasmid#163147PurposeLentiviral gRNA plasmid targeting human CHAT gene, co-expression of BFP tagDepositorInsertCHAT (CHAT Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-mNeonGreen_FRB(DmrC)_IRESpuro2
Plasmid#182918PurposeExpesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2BDepositorInsertpH2B (H2BC16P Human)
UseTagsmNeonGreen-FRB(DmrC)ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-miRFP670-eDHFR(69K6)
Plasmid#178852PurposeMammalian expression of miRFP670-eDHFR(69K6)DepositorInsertmiRFP670-eDHFR(69K6)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-mNG-eDHFR(69K6)
Plasmid#178853PurposeMammalian expression of mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro
Plasmid#169665PurposeTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGK / RPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK9c_2
Plasmid#185023PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK9c
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK9c_1
Plasmid#185022PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK9c
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK10c_2
Plasmid#185021PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK10c
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK10c_1
Plasmid#185020PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK10c
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMETn_2
Plasmid#185019PurposeshRNA mediated knockdownDepositorInsertERV_MMETn
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMETn_1
Plasmid#185018PurposeshRNA mediated knockdownDepositorInsertERV_MMETn
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1021-Tier1-PhCMV-PuroR
Plasmid#169492PurposeTier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)DepositorInsertPCMV-driven puromycin N-acetyl-transferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-mAK1-shRNA-1
Plasmid#185364PurposeFor mammalian expression of shRNA: ATCTTGACTCCCTGAAGTAAC that targets mouse AK1 (adenylate kinase)DepositorInsertAK1 (Ak1 Mouse)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Puro_zhang2.0
Plasmid#167919PurposeLentiviral vector for expressing U6 MS2-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only