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Plasmid#73210PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP)DepositorInsertKozATG-myc-dL5-2XG4S-mCer3-SKL (MYC Synthetic, Human)
UseTagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)
Plasmid#49069PurposeExpresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and mVenusExpressionMammalianMutationC563S,C568S,C577S,C582S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(A73L,L74I,L75S) in pcDNA3.1
Plasmid#42190DepositorInsertG protein Gamma 3(A73L,L74I,L75S) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationA73L,L74I,L75SPromoterCMVAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)
Plasmid#227620PurposeOverexpression of SLC25A46 with R247A and R347A mutations in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
UseTagsP2A-mElectra1ExpressionMammalianMutationR257A,R347APromoterCMV, T7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A/S1346A
Plasmid#215902PurposeExpression of TNRC6A-CIM1 domain with S1332A/S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1528, S1332A/S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP
Plasmid#225490PurposeNeuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseNeuronal expressionTagsExpressionMammalianMutationPromoterhSynAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS
Plasmid#217478PurposeMammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1sDepositorInsertNier1s
UseTags3xNLS, P2A-EGFP-3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS
Plasmid#217479PurposeMammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1bDepositorInsertNier1b
UseTags3xNLS, P2A-EGFP-3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)
Plasmid#201819PurposeA plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoterDepositorInserthChR2(H134R)-V5-ER-LBD (ChRERa)
UseTagsExpressionMammalianMutationPromoterpOTTC1751Available sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS
Plasmid#194751PurposeTo fuse your protein of interest to the C-terminus of GPI-anchored-BioID2DepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-DmRoquin-del725-755-del790-812_AE
Plasmid#148608PurposeMammalian Expression of DmRoquin-del725-755-del790-812DepositorInsertDmRoquin-del725-755-del790-812 (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TagBFP-P2A-Pre-SPHERE-Rncp-iGluSnFR1
Plasmid#187093PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-Rncp-iGluSnFR1
UseTagsTagBFP-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-iRFP-P2A-Post-SPHERE-Rncp-iGluSnFR1
Plasmid#187094PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPost-SPHERE-Rncp-iGluSnFR1
UseTagsiRFP670-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-P2A-Pre-SPHERE-sfmTurquoise2-iGluSnFR
Plasmid#187095PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-sfmTurquoise2-iGluSnFR
UseTagsmCherry-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1461R
Plasmid#164002PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationE1461RPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry W1460A
Plasmid#164001PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationW1460APromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry M1457A
Plasmid#164000PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationM1457APromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry Y1445A
Plasmid#163999PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationY1445APromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1427E
Plasmid#163998PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationR1427EPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1414R
Plasmid#163997PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationE1414RPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only