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pJCC_162 SpCas9 KES(1107-1109)GG

Plasmid

#179526

Purpose

For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 KES(1107-1109)GG

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

replaced KES (residues 1107-1109) with GG

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176237

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Armc12 delta ARM/FLAG

Plasmid

#180496

Purpose

Expression vector of ARM domain deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

Insert

armadillo repeat containing 12 (Armc12 Mouse)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

ARM domain of Armc12 is deleted

Promoter

CAG promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-ABL1-gRNA1-exon4

Plasmid

#163322

Purpose

eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 1_Exon4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)-ABL1-gRNA2-exon4

Plasmid

#163323

Purpose

eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 2_Exon4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

Plasmid

#165082

Purpose

gRNA 1 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

Plasmid

#165083

Purpose

gRNA 2 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

Plasmid

#165085

Purpose

gRNA 1 of pair C for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

Plasmid

#165086

Purpose

gRNA 2 of pair C for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dH85E-cs-TEV-STREP

Plasmid

#171838

Purpose

Mammalian expression of human WIPI2d H85E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep and mCherry

Expression

Mammalian

Mutation

H85E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dK88E-cs-TEV-STREP

Plasmid

#171839

Purpose

Mammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep and mCherry

Expression

Mammalian

Mutation

K88E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dI92E-cs-TEV-STREP

Plasmid

#171840

Purpose

Mammalian expression of human WIPI2d I92E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep and mCherry

Expression

Mammalian

Mutation

I92E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dC93E-cs-TEV-STREP

Plasmid

#171841

Purpose

Mammalian expression of human WIPI2d C93E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep and mCherry

Expression

Mammalian

Mutation

C93E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP

Plasmid

#171842

Purpose

Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep and mCherry

Expression

Mammalian

Mutation

R108E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dR125E-cs-TEV-STREP

Plasmid

#171843

Purpose

Mammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep and mCherry

Expression

Mammalian

Mutation

R125E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP

Plasmid

#171844

Purpose

Mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep and mCherry

Expression

Mammalian

Mutation

K128E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-CHMP3

Plasmid

#154182

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Use

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-retroCHMP3

Plasmid

#154184

Purpose

expresses squirrel monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Use

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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