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pACM4-ACA

Plasmid

#49809

Purpose

Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

ACP transacylase
Acetyl-CoA carboxylase
acetyl-CoA carboxylase
Acetyl-CoA carboxylase
Acetyl-CoA carboxylase

Use

Tags

Expression

Bacterial

Mutation

Silent site-directed mutagenesis to remove SalI s…

Promoter

T7

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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WN10151

Plasmid

#80441

Purpose

Expresses human codon-optimized dead (832A) LbCpf1

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Insert

dead (D832A) LbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

D832A

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-mCherry-N

Plasmid

#210271

Purpose

N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Type

Empty backbone

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3/hArf1(WT)-EGFP

Plasmid

#79413

Purpose

Expresses C-terminally EGFP-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (ARF1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Del 3)

Plasmid

#16603

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Del 3) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Del-3 (AccI->PvuII). The AccI site is at -741 …

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
P447-FBP_19

Plasmid

#139710

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsive

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-DARPinOFF7

Plasmid

#182399

Purpose

Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-DARPinOFF7

Use

Tags

Vesicle Nucleating peptide 15 (VNp15)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SAMTOR

Plasmid

#100508

Purpose

Expresses FLAG tagged SAMTOR in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-ec_bPAC

Plasmid

#28135

Purpose

Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

Depositor

Peter Hegemann

Article

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

E.coli adapted photoactivated adenylyl cyclase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24-C840W

Plasmid

#28136

Depositor

Michelle Barton

Article

Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

Insert

TRIM24 (TRIM24 Human)

Use

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V and C840W

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pRK7 IKK2/IKKb

Plasmid

#26203

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase beta (IKBKB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3/mArf3(T31N)-HA

Plasmid

#79416

Purpose

Expresses C-terminally HA-tagged Arf3(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf3(Q71L)-HA

Plasmid

#79415

Purpose

Expresses C-terminally HA-tagged Arf3(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a WT-ER

Plasmid

#8355

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

mouse estrogen receptor (G525R) on C-term

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB cdc42 WT

Plasmid

#61635

Purpose

mammalian expression of cdc42 wt

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

cdc42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pGLOW31

Plasmid

#172698

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mScarlet

Use

Tags

mScarlet

Expression

Worm

Mutation

Promoter

myo-3

Available since

Aug. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 Flag TSC2 (T1462A)

Plasmid

#14130

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 T1462A (TSC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T1462A

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
Krox20 promoter pGL3-TATA

Plasmid

#21260

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

Krox20 promoter (Egr2 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_VNp-mNeongreen_CydAB-mCherry

Plasmid

#182391

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-mNeongreen-His
cydAB-mCherry

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_StefinA

Plasmid

#182402

Purpose

Bacterial expression of stefinA

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

stefin (CSTA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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