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MEK1C218

Plasmid

#164639

Purpose

Bacterial expression plasmid for His6-MEK1C218

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Use

Tags

His6-tag

Expression

Bacterial

Mutation

G(-19)F/S218C/C277S/C376S

Promoter

T7

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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MAPK9 gRNA (BRDN0001147698)

Plasmid

#76718

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK9

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK9 (MAPK9 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK11 gRNA (BRDN0001147606)

Plasmid

#77482

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK11

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK11 (MAPK11 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK11 gRNA (BRDN0001146070)

Plasmid

#77483

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK11

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK11 (MAPK11 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MK08_HUMAN_D0

Plasmid

#79695

Purpose

This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK08 (MAPK8 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAP2K1 gRNA (BRDN0001146127)

Plasmid

#76213

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K1 (MAP2K1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K2 gRNA (BRDN0001146932)

Plasmid

#77602

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K2 (MAP2K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK12 gRNA (BRDN0001146907)

Plasmid

#77485

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK12

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK12 (MAPK12 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK12 gRNA (BRDN0001148543)

Plasmid

#77486

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK12

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK12 (MAPK12 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK12 gRNA (BRDN0001147863)

Plasmid

#77487

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK12

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK12 (MAPK12 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K3 gRNA (BRDN0001147239)

Plasmid

#76381

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K3 (MAP2K3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK7 gRNA (BRDN0001148313)

Plasmid

#76894

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK7

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK7 (MAPK7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK7 gRNA (BRDN0001145201)

Plasmid

#76895

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK7

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK7 (MAPK7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK7 gRNA (BRDN0001145053)

Plasmid

#76896

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK7

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK7 (MAPK7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK10 gRNA (BRDN0001148192)

Plasmid

#75981

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK10

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK10 (MAPK10 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP3K9 gRNA (BRDN0001146157)

Plasmid

#77309

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K9

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K9 (MAP3K9 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K7 gRNA (BRDN0001149362)

Plasmid

#77820

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K7 gRNA (BRDN0001162319)

Plasmid

#77821

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K7 gRNA (BRDN0001149491)

Plasmid

#77822

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K7 gRNA (BRDN0001162371)

Plasmid

#77823

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge