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pCMV5-Flag-EPLIN alpha delta domain 7

Plasmid

#40940

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha delta Domain 7 (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 562-600

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta domain 3

Plasmid

#40937

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha delta Domain 3 (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 106-148

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN beta delta domain 3

Plasmid

#40939

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta delta domain 3 (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 266-309

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pAG227 CORO1C 3'UTR mut

Plasmid

#12030

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

CORO1C 3'UTR mut (CORO1C Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG83 LRRK1 3'UTR mut

Plasmid

#12058

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N4 3'UTR mut (LRRK1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B2-HA

Plasmid

#209227

Purpose

Mammalian expression of ATP8B2

Depositor

Hye-Won Shin

Article

Takatsu et al Mol Cell Biol. 2024 Sep 2:1-16. doi: 10.

Insert

ATP8B2 (ATP8B2 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg1

Plasmid

#198566

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg1 (SPNS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg2

Plasmid

#198567

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg2 (SPNS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLINE1ΔORF1-2-scFvFc

Plasmid

#206022

Purpose

The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLINE1mORF1-ΔORF2-scFvFc

Plasmid

#206023

Purpose

The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDIV313

Plasmid

#204956

Purpose

AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
hph (hygromycin resistance marker)
sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

Promoter

Aspergillus fumigatus U3 promoter, Aspergillus ni…

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pACE-K2P4.1-StrepTagII

Plasmid

#191474

Purpose

Expresses K2P4.1 with C-terminal StrepTagII

Depositor

Arthur Laganowsky

Article

Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

Insert

K2P4.1 (KCNK4 Human)

Use

Tags

Expression

Insect

Mutation

N104Q N108Q

Promoter

polH

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS2155

Plasmid

#141065

Purpose

AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

Insert

SYN1-EmGFP-WPRE

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

April 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

Plasmid

#166854

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

F641E, I645E

Promoter

pCu

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

Plasmid

#166853

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

F641G, I645G

Promoter

pCu

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOK3_IRS

Plasmid

#109885

Purpose

Protein expression and purification of DOK3_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DOK3_IRS (DOK3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAB_14-3-3

Plasmid

#109904

Purpose

Protein expression and purification of YWHAB_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAB_14-3-3 (YWHAB Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOK2_IRS

Plasmid

#109884

Purpose

Protein expression and purification of DOK2_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DOK2_IRS (DOK2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_HOMER3_WH1

Plasmid

#109891

Purpose

Protein expression and purification of HOMER3_WH1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HOMER3_WH1 (HOMER3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PREX2_DEP

Plasmid

#109893

Purpose

Protein expression and purification of PREX2_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PREX2_DEP (PREX2 Human)

Use

Tags

Expression

Bacterial

Mutation

amino acids 369-474

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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