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We narrowed to 3,281 results for: mak

Showing: 2081 - 2100 of 3281 results

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pHIV-EF1a-Il1a-ZsGreen

Plasmid

#231987

Purpose

Express IL-1alpha-ZsGreen fusion protein

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

IL-1alpha-ZsGreen fusion construct

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-Spo20(PABD)

Plasmid

#221758

Purpose

Stable expression of GFP-Spo20(PABD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Spo20 (SOS1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem Spo20(PABD) (amino acids 422-551)

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIB-mRuby3-LC3

Plasmid

#221738

Purpose

Stable expression of mRuby3-LC3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

LC3B (Map1lc3b Rat)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

Plasmid

#208872

Purpose

For expression in E. coli of NAP1 (delta-NDP52)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Use

Tags

MBP-TEV

Expression

Bacterial

Mutation

S37K/A44E (disrupts NDP52 binding)

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

Plasmid

#208873

Purpose

For expression in E. coli of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Use

Tags

MBP-TEV

Expression

Bacterial

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)

Plasmid

#208863

Purpose

Stably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

S37K/A44E (disrupts NDP52 binding)

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)

Plasmid

#208864

Purpose

Stably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

I11S/L12S (disrupts FIP200 binding)

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208865

Purpose

Stably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R)-GFP

Plasmid

#212793

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R)-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

CUb(K48R)

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K63R)-GFP

Plasmid

#212794

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K63R)-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

CUb(K63R)

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-E3(48) aa24-150-VSV

Plasmid

#212747

Purpose

Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48) aa24-150

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Cue1 ΔU7BD

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

Plasmid

#212738

Purpose

Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

HOIP(C885A)

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

Plasmid

#212805

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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phROSA26-Tet-HA-JLP_WT

Plasmid

#208770

Purpose

Tetracycline inducible expression vector for HA-tagged wild-type JLP protein (HA-JLP_WT), used as a donor plasmid for HA-JLP_WT knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JLP (SPAG9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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phROSA26-Tet-HA-JLP_V55Q

Plasmid

#208771

Purpose

Tetracycline inducible expression vector for HA-JLP_V55Q mutant protein, used as a donor plasmid for HA-JLP_V55Q knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JLP (SPAG9 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LARP1_323-509

Plasmid

#209800

Purpose

Bacterial expression of human LARP1 La-module

Depositor

Kalle Gehring

Article

Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

Insert

LARP1 La-module (LARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 323-509 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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LARP1_323-410

Plasmid

#209801

Purpose

Bacterial expression of human LARP1 LaM domain

Depositor

Kalle Gehring

Article

Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

Insert

LARP1 LaM (LARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 323-410 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Xpress-GBF1(E794K)

Plasmid

#206418

Purpose

Mammalian expression of hGBF1(E794K)

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Use

Tags

Expression

Mammalian

Mutation

E794K

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(Δ1-198)

Plasmid

#186222

Purpose

For expression of ATG13(Δ1-198) tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deleted amino acids 1 - 198

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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