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7432 Bicistronic_GFP_ires_puro

Plasmid

#64336

Purpose

This a retroviral expression plasmid expressing GFP along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

GFP

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Macro-Linker-eGFP

Plasmid

#176067

Purpose

EGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

Macro

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)

Plasmid

#39858

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

C11orf51 (ANAPC15 Human)

Use

Tags

IRES2-mRuby

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pmCherry-miR-125b-1

Plasmid

#58990

Purpose

MSCV based expression vector - co-expresses mCherry and human miR-125b-1

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Inserts

mCherry fluorescent protein
miR-125b-1 (MIR125B1 Human)

Use

Retroviral; Mscv-based retroviral system

Tags

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[3]-C (LEU2)

Plasmid

#177796

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Use

Tags

DHFR F[3]

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-PolB-Hygro

Plasmid

#176056

Purpose

EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-Idax WT

Plasmid

#68553

Purpose

This plasmid expresses full length IDAX.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

IDAX (Cxxc4 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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Sema3f(L,+)-Fc-His

Plasmid

#72147

Purpose

Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNTKV- Utf1-KI-3'UTR-2A-tdTomato-RBGpA

Plasmid

#128834

Purpose

Knock-in of tdTomato into mouse Utf1 locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tdTomato into Utf1 locus (Utf1 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO10-pcDNA3.1-

Plasmid

#52508

Purpose

expresses human FbxO10 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO10 (FBXO10 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-WWE-Linker-eGFP

Plasmid

#176072

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

WWE

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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FusionRed-Dectin1A-N-10

Plasmid

#56112

Purpose

Localization: Membrane, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Dectin1A (CLEC7A Human)

Use

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pX330-Utf1 gRNA

Plasmid

#128842

Purpose

gRNA for targeting mouse Utf1 locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Utf1 gRNA (Utf1 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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ABE8.13-d

Plasmid

#136297

Purpose

expresses ABE8.13-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.13-d

Use

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

Plasmid

#39859

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

C11orf51 (ANAPC15 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
mIFP12-H2B-C-10

Plasmid

#56254

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H2B (H2BC11 Human)

Use

Tags

mIFP12

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-Endo-14

Plasmid

#56250

Purpose

Localization: Endosomes, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Endo (RHOB Human)

Use

Tags

Myc and mIFP12

Expression

Mammalian

Mutation

Deleted amino acids 1-4

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGL3-IRES-miR34a-P2

Plasmid

#64786

Purpose

Luciferase expression driven by miR-34a promoter P2

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P2 (MIR34A Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-BLMP-1-pcDNA3.1-

Plasmid

#52514

Purpose

expresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

blmp-1 (blmp-1 Nematode)

Use

Tags

6xHis

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4D RNAiR

Plasmid

#51600

Purpose

expresses RNAi-resistant mouse Sema4D with N-terminal myc tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D (Sema4d Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

silent mutations between amino acids (approx 275-…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only