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Plasmid#115254PurposeFor mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsMYC-DYKExpressionMammalianMutationC302SPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-MYC-DYK-C302A
Plasmid#115255PurposeFor mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsMYC-DYKExpressionMammalianMutationC302APromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-MYC-DYK-N328A
Plasmid#115246PurposeFor mammalian expression of a human NODAL splice variant open reading frame (with a mutated N-glycosylation site) with a C-terminal MYC-DYK tagDepositorInsertNODAL splice variant (NODAL Human)
UseTagsMYC-DYKExpressionMammalianMutationN328APromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCxcl1-Firefly-1-Tbx5-site
Plasmid#177832PurposeLuciferase reporter for mouse Cxcl1 with one Tbx5 consensus binding siteDepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterCxcl1Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCxcl1-Firefly-2-Tbx5-sites
Plasmid#177833PurposeLuciferase reporter for mouse Cxcl1 with two Tbx5 consensus binding sitesDepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterCxcl1Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP humanAPOBEC1 L173A/G227A
Plasmid#167171PurposeExpresses human APOBEC1 L173A/G227A in mammalian cellsDepositorArticleInserthumanAPOBEC1 (APOBEC1 Human)
UseTagsExpressionMammalianMutationchanged Leucine 173 to Alanine; changed Glycine 2…PromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pChantico-Tdel-Firefly
Plasmid#163902PurposeLuciferase reporter for Chantico lncRNA with Tbx5 site deletionDepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterChantico with Tbx5 consensus binding site deletionAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_drHUR-Halo
Plasmid#175315PurposeProduction of HaloTag fusion proteinDepositorInsertHUR (elavl1a Zebrafish)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_DNAJA1-Halo
Plasmid#175344PurposeProduction of HaloTag fusion proteinDepositorInsertDNAJA1 (DNAJA1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP3_R525C-Halo
Plasmid#175320PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP3 (IGF2BP3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationR525CPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP3_I474M-Halo
Plasmid#175319PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP3 (IGF2BP3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationI474MPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP1_R167H-Halo
Plasmid#175317PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP1 (IGF2BP1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationR167HPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP1_R167C-Halo
Plasmid#175316PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP1 (IGF2BP1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationR167CPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTN177E-myc
Plasmid#107474PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation N177E with approximately wildtype activityDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationR596QPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTY289I-myc
Plasmid#107480PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y289I with approximately wildtype activityDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationN177EPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTK37Q-myc
Plasmid#107476PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K37Q approximately wildtype activityDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationK37QPromoterCMVAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH861-gst-optec P210T
Plasmid#89595PurposePlasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase P210T mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged proline 210 to threoninePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH846-gst-dissc
Plasmid#96907PurposePlasmid for yeast expression of GST encoded by non-preferred codonsDepositorArticleInsertyeast codon dis-optimised glutathione-S transferase
UseTags8xhistidineExpressionYeastMutationPromoterTDH3Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH860-gst-optec A180Q
Plasmid#89594PurposePlasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged alanine 180 to glutaminePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH858-gst-optec V156E
Plasmid#89592PurposePlasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase V156E mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged valine 156 to glutamic acidPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only