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Plasmid#200660PurposeConstruct for chemogenetically silencing and visualizing subpopulations of neurons in the retinaDepositorInsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE
Plasmid#135391PurposeCre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.DepositorInsertTeLC-P2A-EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCambia-loxKan
Plasmid#199590Purposebasic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibilityDepositorInsertFRT-lox66-NosP-KanR-NosT-lox71-FRT
UseCre/LoxTagsExpressionPlantMutationPromoterNosAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
PGKneolox2DTA
Plasmid#13443DepositorInsertPGKneolox2DTA
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-flex-iGABASnFR2-WPRE
Plasmid#218880PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-flex-iGABASnFR2n-WPRE
Plasmid#218878PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F104H R168PPromoterSynapsinAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-DIO-TVA-2A-oG
Plasmid#178431PurposeExpresses TVA and optimized Rabies G (oG) protein in a FLEX cassetteDepositorInsertsTVA950
Optimized G
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationPromoterhSynAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVTH
Plasmid#12262DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLP-mOlig2-ires-gfp
Plasmid#49399Purposemammalian bicistronic expression of mouse Olig2 and EGFPDepositorInsertOlig2 (Olig2 Mouse)
UseCre/Lox; Acceptor vectorTagsEGFP and IRESExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-DIO-TC66T-2A-oG
Plasmid#178430PurposeExpresses TVA(TC66T) and optimized Rabies G (oG) protein in a FLEX cassetteDepositorInsertsTVA950 Glu66→Thr
Optimized G
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationPromoterhSynAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-flex-iGABASnFR2n-WPRE
Plasmid#218881PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
p159-RV-syn-dio-mScarlet-T2A
Plasmid#185700PurposeRetroviral vector expression cre-dependent membrane tagged mScarlet-T2A driven by the rat Synapsin promoter (with XhoI and PacI at 3'end of T2A to clone a gene of interest)DepositorInsertmScarlet
UseRetroviralTagsmScarlet palmitoylation with T2A on 3'-endExpressionMutationPromoterSynapsinAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSIIKS_HIS3_I
Plasmid#109062Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.DepositorInsertHIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pW17
Plasmid#158207Purpose2lox landing pad plasmid with mariner transposon and transposase used for CRAGEDepositorInsertloxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
UseTagsExpressionMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_Cas9-blast_GFP
Plasmid#162074Purpose3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistanceDepositorInsertsCas9-2a-blasticidin S deaminase
EGFP
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEF1a and sv40Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8s-WPRE
Plasmid#162377PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE
Plasmid#162380PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterCAGAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE
Plasmid#162381PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationA25G F286YPromoterCAGAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8m-WPRE
Plasmid#162378PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationA25G F286YPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.LSL.EGFP
Plasmid#100047PurposeAAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only