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Plasmid#211617PurposeRhno1 Cdk1mut7ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsExpressionMammalianMutationS58A, S98A, S133A, T159A, T198A, S191A, and T202APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)
Plasmid#200977PurposeMammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)DepositorInsertEFNB2 (EFNB2 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationD62Q-Q130L-V167L (increase specificity towards he…PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-bio-myc-mCE 211-597
Plasmid#112716PurposeExpresses N-terminally bio-myc-tagged mouse CE/Rngtt 211-597 in mammalian cellsDepositorInsertRngtt (Rngtt Mouse)
UseTagsbiotinylation signal peptide (Tagwerker et al. 20…ExpressionMammalianMutationdeleted amino acids 2-210 (triphosphatase domain)PromoterCMVAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA
Plasmid#184549PurposeRetroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tagDepositorUseRetroviralTags3xFLAG and HA tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1663 - pAAV SYN1 mGas6-Myc-DDK
Plasmid#202540PurposeAn adeno-associated viral vector expressing murine Gas6 fused Myc and DDK epitopes from a synapsin promoterDepositorInsertGas6 (Gas6 Mouse)
UseAAVTagsMyc-DDKExpressionMutationPromoterSYN1Available sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI_mini-NtA-Fs1-mSA-Myc-His
Plasmid#198136PurposeExpress NtA-Fs1 in mammalian cellsDepositorInsertNtA-Fs1
UseTagsmSA, TEV-Myc-HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI_E01_NtA-Fs1-mIgG1Fc-Myc-His-WPRE
Plasmid#198134PurposeExpress NtA-Fs1 in mammalian cellsDepositorInsertNtA-Fs1
UseTagsmIgG1Fc, Myc, HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Myc-FBW7 WD40 (All deleted)
Plasmid#197455PurposeThe plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting.DepositorInsertFBW7 (FBXW7 Human)
UseTagsMycExpressionMammalianMutationFBW& dimerization domain deleted.PromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF – ubiquitin – LEU – 4MYC
Plasmid#193314PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4MYC N-terminal tagging
UseTags4MYCExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
HYG-TET-OFF – ubiquitin – LEU – 4MYC
Plasmid#193316PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4MYC N-terminal tagging
UseTags4MYCExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOttc1473 - pAAV CaMKII KDELR2-Myc-DDK
Plasmid#192600PurposeAn AAV packaging vector that expresses KDELR2 under control of the CaMKII promoter.DepositorInsertKDELR2 (KDELR2 Human)
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOttc1472 - pAAV CaMKII KDELR1-Myc-DDK
Plasmid#192599PurposeAn AAV packaging vector that expresses KDELR1 under control of the CaMKII promoter.DepositorInsertKDELR1 (KDELR1 Human)
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP229-Lenti-TRE3G-4xMyc-GluN2B-WPRE-pA
Plasmid#164794PurposepLenti vector backbone designed to express 4XMyc Tagged-GluN2B from a TRE3G promoter.DepositorInsertGluN2B
UseLentiviralTags4xMycExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
Plasmid#172599PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.DepositorInsertmyc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842V+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter
Plasmid#172600PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.DepositorInsertmyc-PDGFRA-K627R+R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627R+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-BirA-Linker-Myc-mRap1
Plasmid#184644PurposeRetroviral expression of BirA-mRap1DepositorInsertBirA-rap1
UseRetroviralTagsBirA FLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-myc-SBP-DmDCP2-E361A-dsRNAres_J
Plasmid#146630PurposeInsect Expression of DmDCP2-E361A-dsRNAresDepositorInsertDmDCP2-E361A-dsRNAres (DCP2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-myc-SBP-DmDCP2-E361Q-dsRNAres_J
Plasmid#146631PurposeInsect Expression of DmDCP2-E361Q-dsRNAresDepositorInsertDmDCP2-E361Q-dsRNAres (DCP2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M
Plasmid#140454PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, tyrosine 616 to methioni…PromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N
Plasmid#140455PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, leucine 624 to asparaginePromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only