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Showing: 4081 - 4120 of 148587 results

  1. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid
    #60365
    Purpose
    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI
    Use
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60365-plasmid-map-sequence-id-256737

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  8. pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

    Plasmid
    #70231
    Purpose
    ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope
    Depositor
    Steven Henikoff
    Article
    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.
    Insert
    3xFLAG tag and aa 83-231 of Mnase
    Use
    Yeast genomic targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70231-plasmid-map-sequence-id-129162

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  12. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid
    #170185
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.
    Depositor
    Hunter Fraser
    Article
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Inserts
    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and hU6
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    170185-plasmid-map-sequence-id-333571

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  15. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

    Plasmid
    #196415
    Purpose
    Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smMyc
    Use
    AAV; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and GfaABC1D
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196415-plasmid-map-sequence-id-385068

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  17. RAB1A

    Plasmid
    #25521
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB1A (RAB1A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. Flag-neo-PP1-Beta

    Plasmid
    #171269
    Purpose
    Mammalian expression of 3x-Flag-tagged human PP1 beta catalytic subunit
    Depositor
    Steven Balk
    Insert
    3x-Flag-tagged human PP1 beta catalytic subunit (PPP1CB Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171269-plasmid-map-sequence-id-335788

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  22. CHRM3-Tango

    Plasmid
    #66250
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CHRM3 (CHRM3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

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  24. pET-29b(+)_His-CBD-SpEndoH

    Plasmid
    #218196
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
    Depositor
    Florence Pojer
    Insert
    EndoH
    Use
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-313
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218196-plasmid-map-sequence-id-431799

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  32. pAAV-STAT-CA

    Plasmid
    #195576
    Purpose
    Expresses STAT3 constitutively active mutant
    Depositor
    Kevin Park
    Article
    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi
    Insert
    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    changed Alanine 662 to Cysteine and Asparagine 66…
    Promoter
    CMV enhancer and promoter
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195576-plasmid-map-sequence-id-383906

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Showing: 4081 - 4120 of 148587 results