Addgene

Showing: 2021 - 2040 of 5212 results

Results per page:

Sort results by:

pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

Plasmid

#221552

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

gRNA targeting human Rab7A (RAB7A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJS-BCD-Strep-PCaspase-R153A

Plasmid

#214036

Purpose

Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

R153A

Promoter

T7 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

Plasmid

#213728

Purpose

Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bIV spectrin (repeats 13-15) (Sptbn4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-3xFLAG-bII spectrin (repeats 13-15)

Plasmid

#213729

Purpose

Mammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bII spectrin (repeats 13-15) (Spnb3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGG-GST-TEV-mCherry-NAP1 (WT)

Plasmid

#198036

Purpose

Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Use

Tags

GST-TEV and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCaUra3-CUbo(K48R/K63R)-yeGFP

Plasmid

#212774

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-GST-ATG13 (363-450)

Plasmid

#210849

Purpose

Mammalian expression of ATG13 (363-450) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-FIP200 (1-640)-R334D-TSF

Plasmid

#210841

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

R334D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-FIP200 (1-640)-R326D-TSF

Plasmid

#210840

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

R326D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-FIP200 (1-640)-V314D-TSF

Plasmid

#210839

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

V314D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-FIP200 (1-640)-V44D-TSF

Plasmid

#210843

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

V44D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-B-HypaR-SpCas9-NLS-6xHis

Plasmid

#207388

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A, amino acids 10…

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-SpCas9-HF1-NLS-6xHis

Plasmid

#207382

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-B-HiFi SpCas9-NLS-6xHis

Plasmid

#207378

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-B-Sniper SpCas9-NLS-6xHis

Plasmid

#207377

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-Blackjack SpCas9-NLS-6xHis

Plasmid

#207375

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Blackjack SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-GST-ATG13 (363-460)

Plasmid

#203553

Purpose

Mammalian expression of GST tagged ATG13 (363-460)

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-SMSr-TEV-Twin-Strep

Plasmid

#202530

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Insert

SAMD8 (SAMD8 Human)

Use

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

deleted stop codon (TGA)

Promoter

CAG and chicken β-actin promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-ATG13 (363-517)-MBP

Plasmid

#203561

Purpose

Mammalian expression of ATG13 (363-517) with MBP tag

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

MBP-10His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge