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Showing: 2021 - 2040 of 2066 results

  5. pDule-3-nitroTyrosine (5B)

    Plasmid
    #85498
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85498-plasmid-map-sequence-id-169862

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  6. pGLACTE-PseudoHT51

    Plasmid
    #99257
    Purpose
    Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresis
    Depositor
    Karen Usdin
    Article
    Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi
    Insert
    FMR1 (FMR1 Human, Synthetic)
    Use
    Tags
    Expression
    Mutation
    Contains synthetic, non-human, primer sequence do…
    Promoter
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    99257-plasmid-map-sequence-id-181641

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  7. pDule2-3-nitroTyrosine (5B)

    Plasmid
    #85499
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85499-plasmid-map-sequence-id-169856

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  10. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

    Plasmid
    #140014
    Purpose
    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021
    Insert
    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y126A, Y197F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140014-plasmid-map-sequence-id-297743

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  12. pRSF-MjCouRS

    Plasmid
    #229066
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024
    Insert
    Methanococcus jannaschii tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    229066-plasmid-map-sequence-id-459819

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  16. pDule2 - Mj Acd A9

    Plasmid
    #197652
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Ryan Mehl
    Article
    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197652-plasmid-map-sequence-id-388215

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  17. pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

    Plasmid
    #154774
    Purpose
    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    in PylT: UUA anticodon and G10A, G14U, G16U, C20U…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154774-plasmid-map-sequence-id-310423

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Showing: 2021 - 2040 of 2066 results