-
Plasmid#189735PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204343PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i3/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2345_BTB2x-mCherry
Plasmid#215097PurposeExpresses BCL6 BTB(1-129)-BTB(1-129)-mCherry in mammalian cellsDepositorInsertBCL6 BTB-BTB (BCL6 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP775_αGCN4-p65HSF1-CO
Plasmid#211768PurposeSunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selectionDepositorInsertaGCN4-p65HSF1
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMEH343 CD63 MS2 HA
Plasmid#168217PurposeMammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tagDepositorInsertCD63 (CD63 Human)
UseTagsHA and MS2 dimerExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
THBS1-bio-His
Plasmid#53417PurposeExpresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTHBS1 (THBS1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTL5
Plasmid#127967PurposeDHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTL3
Plasmid#127966Purposeconstitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SynTetOff-GFP
Plasmid#190231PurposeAAV vector, high-level expression of EGFP in neuronal cellsDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PME-1 pcDNA-StrepIII
Plasmid#119292PurposeMammalian overexpression of StrepII-tagged full length PPME1DepositorInsertPPME1 (PPME1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI
Plasmid#115267PurposeExpresses LbuCas13a from a SUMO-tagged bacterial expression constructDepositorInsertLbuCas13a
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
JI401: Ad5 E1A/Hexon qRT-PCR standard
Plasmid#131753PurposePlasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).DepositorInsertsAd5 E1A gene fragment
Ad5 Hexon gene fragment
UseStandard for qrt-pcrTagsExpressionMutationPromoterN/AAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sst44-GFP
Plasmid#172310PurposeSST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneuronsDepositorInsertSst44
UseAAVTagsExpressionMutationPromoterpB-GlobinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGCaMP6m-N3-TPC2
Plasmid#80147PurposeGCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real timeDepositorInsertTPCN2 (TPCN2 Human)
UseTagsGCaMP6mExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-EGFP-SOS1
Plasmid#178860PurposeMammalian expression of SOS1 fused to EGFPDepositorInsertSOS1 (SOS1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-FlicR1
Plasmid#74142PurposeMammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicatorDepositorInsertFlicR1
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6sgp53-U6sgbbSapI-GFAPCre
Plasmid#100275PurposeAAV-CRISPR mediated genome editing in glia / astrocytesDepositorTypeEmpty backboneUseAAV, CRISPR, Mouse Targeting, and Synthetic Biolo…TagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i2 ONE-GO
Plasmid#189736PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTDH-His-bio
Plasmid#52331PurposeExpresses full-length Metastasis adhesion protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertMTDH (MTDH Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only