JP705: pMVP/SB/Neo-DEST
(Plasmid
#121866)
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Purpose(Empty Backbone) pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassette
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121866 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneJN607
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Backbone manufacturerNewgard Lab
- Backbone size (bp) 4657
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Vector typeMammalian Expression ; Sleeping Beauty Transposon, pMVP Gateway Destination Vector
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGateway selection cassette (ccdb gene + Chlor resistance gene)
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SpeciesSynthetic
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Insert Size (bp)1455
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sleeping Beauty ITR sequences derived from Addgene #26563
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JP705: pMVP/SB/Neo-DEST was a gift from Christopher Newgard (Addgene plasmid # 121866 ; http://n2t.net/addgene:121866 ; RRID:Addgene_121866) -
For your References section:
Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. 10.1093/nar/gky1286 PubMed 30590691