Addgene

Showing: 2001 - 2020 of 4918 results

Results per page:

Sort results by:

pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1427E

Plasmid

#163986

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R1427E

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1414R

Plasmid

#163985

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E1414R

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1411R

Plasmid

#163984

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E1411R

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG D1401R

Plasmid

#163983

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R1401R

Promoter

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1397R

Plasmid

#163982

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E1397R

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1392E

Plasmid

#163981

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R1392E

Promoter

Available since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1386E

Plasmid

#163980

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R1386E

Promoter

Available since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

Plasmid

#131255

Purpose

Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)

Plasmid

#72889

Purpose

fusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

Inserts

Gaussia luciferase
Volvox channelrhodopsin 1
Enhanced Yellow Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127317

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG

Plasmid

#127325

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PEST

Plasmid

#127320

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127321

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/AUU-nLuc-3XFLAG-CL1/PEST

Plasmid

#127319

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUU start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG

Plasmid

#127324

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/BAG1 5′ UTR AUG-nLuc-3xFLAG

Plasmid

#127329

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with BAG1 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/BAG1 5′ UTR CUG-nLuc-3xFLAG

Plasmid

#127328

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon from mRNA with BAG1 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1(-) + SLNCR1 372-672 (conserved sequence)

Plasmid

#86826

Purpose

Expression of conserved region of lncRNA SLNCR1

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-847

Plasmid

#69902

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 3xFLAG TPP1 88-249 delta166-172

Plasmid

#53550

Purpose

mammalian expression of TPP1 OB-fold domain with 166-172 replaced with "GSSG"

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

contains aa 88-249, with aa 166-172 replaced with…

Promoter

CMV

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only