Addgene

We narrowed to 4,003 results for: gast

Showing: 2001 - 2020 of 4003 results

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pAc5.1B-lambdaN-HA-DmHPat_1-499_A

Plasmid

#145876

Purpose

Insect Expression of DmHPat_1-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_1-499 (Patr-1 Fly)

Use

Tags

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmXRN1_A

Plasmid

#145879

Purpose

Insect Expression of DmXRN1

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmXRN1 (pcm Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Promoterless TP10

Plasmid

#165787

Purpose

Drosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectors

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Promoterless T10

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-pleCopyCatcher

Plasmid

#174061

Purpose

CopyCatcher insertion donor for the pale locus

Depositor

Ethan Bier

Article

Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

Insert

Pale (Pale Fly, Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMe31B_268-459_D

Plasmid

#146119

Purpose

Insect Expression of DmMe31B_268-459

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmMe31B_268-459 (me31B Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1052B (Ubiq-Fluc_Ubiq-Rluc)

Plasmid

#132426

Purpose

Express Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

Ubiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pKM44

Plasmid

#154153

Purpose

Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Drosophila melanogaster minimal Hsp70 promoter

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-CMV

Plasmid

#133366

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Use

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-CMV-Luc

Plasmid

#133367

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Use

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PIR702W-Luc

Plasmid

#133362

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IRFP-702

Use

Tags

HA-tagged IRFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PIR702W

Plasmid

#133361

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IRFP-702

Use

Tags

HA-tagged IRFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-Luc

Plasmid

#133360

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Use

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS attB V5 dDispatched WT HA

Plasmid

#126411

Purpose

Expression of wild type dDispatched HA in Drosophila.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-attB V5 dDispatched delta CS HA

Plasmid

#126412

Purpose

Expression of V5 dDispatched Delta CS HA in Drosophila cells lines

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Use

Tags

HA

Expression

Insect

Mutation

Disp1 Cleavage site

Promoter

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTGC-U6.3

Plasmid

#112810

Purpose

PCR template vector for amplifying gRNAcore-pU6.2 promoter fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

U6:3-gRNAcore

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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GST-GNU-N

Plasmid

#112972

Purpose

Expresses fusion of GST and N terminal amino acids of GNU

Depositor

Terry Orr-Weaver

Article

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP 9A

Plasmid

#113006

Purpose

P element vector for transposon insertion of gnu-GFP 9A into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

9 CycB/CDK1 sites mutated to A

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP WT

Plasmid

#113005

Purpose

P element vector for transposon insertion of gnu-GFP WT into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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6xmyc_cort_UAS-P

Plasmid

#112991

Purpose

P element vector for transposon insertion of Myc-tagged Drosophila cort (Cdc20) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Pesin et al PLoS Genet. 2007 Nov;3(11):e202. doi: 10

Insert

cort (Cdc20) (cort Fly)

Use

P insertion vector

Tags

6X myc epitope

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C-term png in pGEX4

Plasmid

#112975

Purpose

For protein expression and purification of the C-term of Drosophila png

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png (png Fly)

Use

Tags

Expression

Bacterial

Mutation

wild-type C terminus

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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