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Showing: 2001 - 2020 of 5979 results

  1. pODN-AAVS1-mR2-CAAX

    Plasmid
    #183871
    Purpose
    Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183871-plasmid-map-sequence-id-373475

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  5. pAAV-EF1a-DIO-HTB

    Plasmid
    #44187
    Depositor
    Edward Callaway
    Insert
    Histone tagged GFP, T2A-TVA-E2A-B19G
    Use
    AAV; Adeno-associated vector
    Tags
    GFP
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 14, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV-tMCK-mem-TurboID

    Plasmid
    #149410
    Purpose
    Muscle-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection
    Depositor
    Jonathan Long
    Insert
    mem-TurboID
    Use
    AAV
    Tags
    HA and V5
    Expression
    Mutation
    Promoter
    tMCK
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149410-plasmid-map-sequence-id-299812

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  10. AAV-CAG-K-red-SPOTIT

    Plasmid
    #191490
    Purpose
    Red fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-red-SPOTIT
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191490-plasmid-map-sequence-id-377865

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  11. AAV-CAG-M-red-SPOTIT2

    Plasmid
    #191491
    Purpose
    Red fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    M-red-SPOTIT2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191491-plasmid-map-sequence-id-377934

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  16. AAV-KrasG13C/sgKras/Cre

    Plasmid
    #99856
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99856-plasmid-map-sequence-id-193794

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  19. pAAV2-CAG-mGreenLantern-KASH

    Plasmid
    #191094
    Purpose
    To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Pantelis Tsoulfas, Murray Blackmore
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mGreenLantern-KASH
    Use
    AAV and Synthetic Biology
    Tags
    mGreenLantern fused to the KASH domain of Nesprin…
    Expression
    Mammalian
    Mutation
    Optimizzation was done using the Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191094-plasmid-map-sequence-id-380125

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Showing: 2001 - 2020 of 5979 results