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pAAV-FLEX-SaCas9-U6-sgKcnab2

Plasmid

#192795

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcnab2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcnab2

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP223-AAV-EFS-GFP-pA

Plasmid

#62913

Purpose

AAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

short form of Elongation Factor1α (EFS)

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FlpE-bGHpA

Plasmid

#50364

Purpose

Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

FlpE recombinase gene

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGabrg1

Plasmid

#124856

Purpose

Mutagenesis of Gabrg1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg1 (Gabrg1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV syn Arrestin-TEVp-V5

Plasmid

#173118

Purpose

Expresses arrestin receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp-V5

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-KrasG13D/sgKras/Cre

Plasmid

#99857

Purpose

Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

Depositor

Monte Winslow

Article

Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

Insert

Kras (Kras Mouse)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

Promoter

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChrimsonR-LSSmOrange

Plasmid

#149691

Purpose

For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-rev-g2-2A-Venus

Plasmid

#71410

Purpose

This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.

Depositor

William Wisden

Article

Uygun et al J Neurosci. 2016 Nov 2;36(44):11171-1118

Inserts

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

mouse hdc gene promoter, works as a pan neuronal …

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRger3-TS-EYFP

Plasmid

#127241

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger3-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Mutation

Promoter

CamKIIa

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

Plasmid

#124864

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FDIO-SYP-HRP

Plasmid

#117187

Purpose

Plasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

SYP-HRP

Use

AAV; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Chronos-GFP

Plasmid

#122098

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1α (1.1 kb short version)

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1alpha-soCoChR-GFP

Plasmid

#107709

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA

Plasmid

#47453

Purpose

epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE (N136,Grm2,C63)-cib1

Use

AAV; Tale

Tags

HA

Expression

Mammalian

Mutation

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-HDR-sphys-3XHA

Plasmid

#163690

Purpose

plasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin gene

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

triple HA tag insertion into endogenous mouse synaptophysin gene

Use

AAV

Tags

triple HA tag

Expression

Mutation

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-KrasG12S/sgKras/Cre

Plasmid

#99853

Purpose

Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

Depositor

Monte Winslow

Article

Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

Insert

Kras (Kras Mouse)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

Promoter

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Mac-GFP

Plasmid

#58853

Purpose

AAV-mediated expression of Mac-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Syn

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-NRSE-RFP

Plasmid

#22929

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

E1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-OTp-hM3Dq-Myc

Plasmid

#184753

Purpose

To drive hM3D fused with Myc tag under the control of mouse oxytocin promoter

Depositor

Kazunari Miyamichi

Article

Inada et al Neuron. 2022 Apr 12. pii: S0896-6273(22)

Insert

hM3D-myc

Use

AAV

Tags

Myc

Expression

Mutation

Promoter

mouse Oxytocin promoter

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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p-AAV2-hSyn-MerMAID6-Citrine

Plasmid

#126521

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID6

Use

AAV

Tags

Citrine

Expression

Mutation

Promoter

human synapsin

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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