-
Plasmid#76271Purpose3rd generation lentiviral gRNA plasmid targeting human GRK5DepositorInsertGRK5 (GRK5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK1 CBM
Plasmid#53196PurposeExpresses HA tagged human MEK1 M187A/H188A/M230A/H239A from puromycin resistance retroviral vectorDepositorInsertMEK1 (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationM187A/H188A/M230A/H239APromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cherry-SRBC
Plasmid#69492PurposeExpression of fluorescently tagged SRBC in mammalian cellsDepositorInsertSRBC (Cavin3 Mouse)
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT MEK5-DD-mRFP1
Plasmid#47547PurposeGateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutantDepositorInsertMEK5-DD (MAP2K5 Human)
UseEntry vectorTagsmRFPExpressionMutationS311D, T315DPromoterAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
GRK7 gRNA (BRDN0001148138)
Plasmid#76501Purpose3rd generation lentiviral gRNA plasmid targeting human GRK7DepositorInsertGRK7 (GRK7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K11 gRNA (BRDN0001146472)
Plasmid#76364Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K11DepositorInsertMAP3K11 (MAP3K11 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K11 gRNA (BRDN0001145849)
Plasmid#76365Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K11DepositorInsertMAP3K11 (MAP3K11 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K11 gRNA (BRDN0001144833)
Plasmid#76366Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K11DepositorInsertMAP3K11 (MAP3K11 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MK11_HUMAN_D0
Plasmid#79692PurposeThis plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK11 (MAPK11 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG AKT3 E17K;K177M
Plasmid#73049PurposeHuman AKT3 E17K;K177MDepositorInsertAKT3 E17K;K177M (AKT3 Human)
UseTagsExpressionMammalianMutationE17K;K177MPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK3_HUMAN_D0
Plasmid#79702PurposeThis plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMAPK3 (MAPK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
GRK5 gRNA (BRDN0001148840)
Plasmid#76274Purpose3rd generation lentiviral gRNA plasmid targeting human GRK5DepositorInsertGRK5 (GRK5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-MEK1-MEK5DD
Plasmid#53177PurposeExpresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vectorDepositorInsertMEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationMEK1 (1-201) MEK5(498–1344 with S311D/T315D)PromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xHA-MKK6-EE
Plasmid#47544PurposeGateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutantDepositorInsert3xHA-MKK6-EE (MAP2K6 Human)
UseEntry vectorTags3XHAExpressionMutationS207E, T211EPromoterAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt2
Plasmid#220240PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseTags6x His, MBPExpressionInsectMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterPolyhedrinAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PICK1_POLR2F
Plasmid#205894PurposeExpress mEGFP-tagged fusion protein, PICK1_POLR2F from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-AKT1
Plasmid#170682Purposedoxycycline-inducible overexpression of AKT1DepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant nmPKA-Cα-mEGFP
Plasmid#114144PurposeThe G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
UseTagsExpressionMammalianMutationG2A mutation and silent mutation to resist shRNA …PromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_WNK3_p.S617F
Plasmid#81709PurposeGateway Donor vector containing WNK3,part of the Target Accelerator Plasmid Collection.DepositorInsertWNK3 (WNK3 Human)
UseGateway entry vectorTagsExpressionMutationS617FPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_WNK3_p.E417Q
Plasmid#81716PurposeGateway Donor vector containing WNK3, part of the Target Accelerator Plasmid Collection.DepositorInsertWNK3 (WNK3 Human)
UseGateway entry vectorTagsExpressionMutationE417QPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only