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Plasmid#159456PurposeDonor empty vector with puromycin resistance gene expression cassette flanked by FRTDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCMV-PE2-tagRFP-BleoR
Plasmid#192508PurposeExpression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.DepositorInsertPE2-P2A-tagRFP-P2A-BleoR
UseTagsBleoR, SV40 bpNLS, and tagRFPExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
AMA1-bio
Plasmid#47741PurposeExpresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised AMA1
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP5.v2 puro
Plasmid#87793PurposeExpresses an inducible short hairpin targeting human MKP5 sequenceDepositorInsertshMKP5 (DUSP10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP5.v1 puro
Plasmid#87792PurposeExpresses an inducible short hairpin targeting human MKP5 sequenceDepositorInsertshMKP5 (DUSP10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR1 DD_NES
Plasmid#124630PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with MCP_ADAR1 DD_NESDepositorInsertMCP_ADAR1 DD_NES (RAB7A Human)
UseAAVTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalA Q75L
Plasmid#19719DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationGTPase deficient & constitutively active Gl…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta LIM
Plasmid#40933DepositorInsertEPLIN alpha (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationLIM domain deleted- amino acids 221-295PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta C-term
Plasmid#40930DepositorInsertEPLIN alpha (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 296-600PromoterAvailable sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA15_G-alpha
Plasmid#109782PurposeProtein expression and purification of GNA15_G-alphaDepositorInsertGNA15_G-alpha (GNA15 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/Ecad-tdTomato
Plasmid#101278PurposeLentiviral expression of shRNA targeting Ecad and Ecad-tdtomatoDepositorInsertE-cadherin
UseLentiviralTagstdtomatoExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro C-Raf 22W
Plasmid#12593DepositorInsertN-term truncated mut of Raf-1 (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutation22W, N-terminally truncatedPromoterAvailable sinceSept. 21, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLInChR-mVenus
Plasmid#119298PurposeFLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitorDepositorInsertNx1BTM-FCS-ChR2ET/TC-mVenus
UseAAVTagsmVenusExpressionMutationPromoterCAGAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP1.v1 puro
Plasmid#87790PurposeExpresses an inducible short hairpin targeting human MKP1 sequenceDepositorInsertshMKP1 (DUSP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE
Plasmid#123360PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.DepositorInsertEmpty LbCpf1 crRNA cloning cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shJUND puro
Plasmid#58698PurposeLentiviral shRNA vector for knockdown of human JUNDDepositorInsertshJUND (JUND Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
EBA140-bio
Plasmid#47742PurposeExpresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised EBA140
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MTRAP-bio
Plasmid#47746PurposeExpresses enzymatically monobiotinylated full-length MTRAP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MTRAP
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FLJ20309
Plasmid#15360DepositorInsertFLJ20309 106-544residues (INO80D Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYLL204
Plasmid#53455PurposeExpresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)InsertHA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)
UseTagsHAExpressionMammalianMutationY814FPromoterCMVAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only