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Plasmid#221475PurposeGateway cloning of NKX2-1 N-terminal truncation mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal domainPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_DelNTerm_160-371AA
Plasmid#221487PurposeNKX2-1 N-terminal truncation mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 1-159AA N-terminal domainPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_V205F
Plasmid#221485PurposeNKX2-1 V205F DNA binding mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationV205FPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-hPGK_NKX2-1_Short_V5-GFP
Plasmid#221498PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterhPGKAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_DelCTerm_1-223AA
Plasmid#221488PurposeNKX2-1 C-terminal truncation mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 224-371AA C-terminal domainPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-hPGK_NKX2-1
Plasmid#221494PurposeNKX2-1 short isoform lentiviral overexpression using hPGK promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterhPGKAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-SFFV_NKX2-1
Plasmid#221496PurposeNKX2-1 short isoform lentiviral overexpression using SFFV promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-UBC_NKX2-1_Short_V5-GFP
Plasmid#221499PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterUBCAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_Short_V5-GFP
Plasmid#221497PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD236
Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL452
Plasmid#219502PurposeGolden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4tDepositorInsertGAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL390
Plasmid#219500PurposeIntegrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpressionDepositorInsertIntegrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT
UseTagsSV40-NLSExpressionYeastMutationPromoterGAL1-GAL10Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta Full (1-190)
Plasmid#99662PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorArticleInsertdCas9
UseTagstruncated RTA activation domain (1-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5(PrD)
Plasmid#166167PurposeGateway entry vector for N. furzeri DDX5-PrD(535-634) mutantDepositorArticleInsertDDX5-PrD(535-634)
UseGateway entry vectorTagsExpressionMutationPrD only 1606-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5(IDR)
Plasmid#166166PurposeGateway entry vector for N. furzeri DDX5-IDR(483-634) mutantDepositorArticleInsertDDX5-IDR(483-634)
UseGateway entry vectorTagsExpressionMutationIDR only 1450-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-ConVERGD-eGFP-WPRE
Plasmid#218749PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-ConVERGD-eGFP-W3SL
Plasmid#218748PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF2 CTF-FLAG
Plasmid#217695PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF2 (ADGRF2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-429PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P
Plasmid#216400PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51 and 56 to Proline, and deleted…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE4 CTF-FLAG
Plasmid#217692PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE4 (ADGRE4P Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-173PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only